Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
State Key Laboratory for Managing Biotic and Chemical Threats To the Quality and Safety of Agro-Products, Hangzhou, 310021, China.
Sci Rep. 2021 Jul 22;11(1):15010. doi: 10.1038/s41598-021-94395-y.
Chilo suppressalis (Walker, 1863) is a serious stem borer of rice and water-oat plants, and has phenotypically diverged into rice and water-oat populations. Insect gut microbiota plays an important role in the host life and understanding the dynamics of this complicated ecosystem may improve its biological control. The effect of diet and gut compartments on the gut microflora of divergent populations of C. suppressalis is not fully clear. Herein, we characterized the gut microbiota of C. suppressalis populations fed on two hosts (i.e., water-oats fruit pulps and rice seedlings), by sequencing the V3-V4 hypervariable region of the 16S rRNA gene using the Illumina MiSeq platform. Gut bacterial communities showed variation in relative abundance among C. suppressalis populations fed on water-oats fruit pulps or rice seedlings. Proteobacteria and Firmicutes became the predominant phyla, and Enterobacteriaceae, Enterococcaceae and Halomonadaceae were the predominant family in all C. suppressalis populations. The highest bacteria diversity was found in the midgut of the rice population fed on water-oat fruit pulps. Bacterial communities in the midgut were more diverse than those in the hindgut. The bacterial genera distribution showed great differences due to diet types and gut compartments among populations. Our results demonstrated that the host plants tested had a considerable impact on gut bacterial composition of C. suppressalis populations. Additionly, the unique gut morphology and physiological conditions (viz., oxygen content, enzymes) also contributed to variation in microbiomes. In conclusion, our study provided an important insight into investigation of insect-bacteria symbioses, and biocontrol of this species and other related lepidopterans.
红棕象甲（Chilo suppressalis（Walker，1863））是一种严重危害水稻和茭白植物的蛀干害虫，其表型已分化为水稻种群和茭白种群。昆虫肠道微生物群在宿主生活中起着重要作用，了解这个复杂生态系统的动态可能会改善其生物防治效果。饮食和肠道隔间对分化种群的肠道微生物群的影响尚不完全清楚。在此，我们通过使用 Illumina MiSeq 平台对 V3-V4 高变区 16S rRNA 基因进行测序，研究了取食于两种宿主（茭白果肉浆和水稻幼苗）的红棕象甲种群的肠道微生物群。肠道细菌群落的相对丰度在取食茭白果肉浆或水稻幼苗的红棕象甲种群之间存在差异。变形菌门和厚壁菌门成为主要的门，肠杆菌科、肠球菌科和盐单胞菌科是所有红棕象甲种群的主要科。在取食茭白果肉浆的水稻种群的中肠中发现了最高的细菌多样性。中肠的细菌多样性高于后肠。由于种群的饮食类型和肠道隔间，细菌属的分布因种群而异。我们的研究结果表明，所测试的宿主植物对红棕象甲种群的肠道细菌组成有很大影响。此外，独特的肠道形态和生理条件（即氧气含量、酶）也导致了微生物组的变异。总之，我们的研究为昆虫-细菌共生关系的研究以及该物种和其他相关鳞翅目昆虫的生物防治提供了重要的见解。
