Khaghani Ramin, Mirzaii-Dizgah Iraj, Ghasemi Mostafa
Department of Medical Parasitology, School of Medicine, Aja University of Medical Sciences, Tehran, Iran.
Department of Physiology, School of Medicine, Aja University of Medical Sciences, Tehran, Iran.
J Arthropod Borne Dis. 2017 May 27;11(2):204-210. eCollection 2017 Jun.
Dermatitis caused by beetle involves many people around the world, especially Iran. The symptoms include redness, itching and severe irritation. This study evaluated the effectiveness of the cream on the treatment of dermatitis caused by beetles.
Forty male 6-8 weeks BALB/C mice were randomly divided into four groups of 10 mice. After removing the mice's back hair, the backs of mice were marked by with a of 3 mm. The beetles were collected from Babol in Mazandaran Province, northern Iran and transferred to the animal lab of Aja University of Medical Sciences, Tehran, Iran. The end of abdominal segment was cut with scissors and hemolymph content was pushed by forceps on the circle. Only hemolymph of one applied to the back of each mouse. Groups 1, 2, 3 and 4 were treated with the base (vehicle), dexamethasone 0.1%, 0.5% and 2% creams respectively. After 2 days, dermatitis appeared. Then the mentioned creams were applied on the mice once a day. The wound area was measured every day. Dermatitis surface area under curve (AUC) of each mouse was calculated for 17 days after induction of dermatitis. Statistical analysis of ANOVA was used.
Application of 0.5% and 2% significantly reduced the healing duration and dermatitis area in comparison with the vehicle and dexamethasone cream (P< 0.05). But dexamethasone had no significant effect on the healing of dermatitis as compared to vehicle.
may clinically effective in the treatment of dermatitis.
甲虫引起的皮炎在世界各地影响着许多人，尤其是在伊朗。症状包括发红、瘙痒和严重刺激。本研究评估了[具体乳膏名称]乳膏对治疗甲虫引起的皮炎的有效性。
40只6 - 8周龄的雄性BALB/C小鼠被随机分为四组，每组10只。去除小鼠背部毛发后，用3毫米的[具体工具名称]在小鼠背部做标记。甲虫从伊朗北部马赞德兰省的巴博勒采集，然后转移到伊朗德黑兰阿亚医科大学的动物实验室。用剪刀剪掉腹部末端节段，并用镊子将血淋巴内容物挤在圆圈上。每只小鼠背部仅涂抹一只甲虫的血淋巴。第1、2、3和4组分别用基质（赋形剂）、0.1%地塞米松、0.5%[具体乳膏名称]和2%[具体乳膏名称]乳膏进行治疗。2天后，出现皮炎。然后每天给小鼠涂抹上述乳膏一次。每天测量伤口面积。计算每只小鼠在诱导皮炎后17天的皮炎曲线下面积（AUC）。采用方差分析进行统计分析。
与赋形剂和地塞米松乳膏相比，应用0.5%和2%[具体乳膏名称]显著缩短了愈合时间并减小了皮炎面积（P < 0.05）。但与赋形剂相比，地塞米松对皮炎愈合没有显著影响。
[具体乳膏名称]在治疗甲虫性皮炎方面可能具有临床疗效。
