Department of Integrative Oncology, British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada V5Z 1L3.
Sci Data. 2017 Oct 24;4:170157. doi: 10.1038/sdata.2017.157.
Only 3% of the transcribed human genome is translated into protein, and small non-coding RNAs from these untranslated regions have demonstrated critical roles in transcriptional and translational regulation of proteins. Here, we provide a resource that will facilitate cell line selection for gene expression studies involving sncRNAs in cancer research. As the most accessible and tractable models of tumours, cancer cell lines are widely used to study cancer development and progression. The NCI-60 panel of 59 cancer cell lines was curated to provide common models for drug screening in 9 tissue types; however, its prominence has extended to use in gene regulation, xenograft models, and beyond. Here, we present the complete small non-coding RNA (sncRNA) transcriptomes of these 59 cancer cell lines. Additionally, we examine the abundance and unique sequences of annotated microRNAs (miRNAs), PIWI-interacting RNAs (piRNAs), small nuclear RNAs (snRNAs), and small nucleolar RNAs (snoRNAs), and reveal novel unannotated microRNA sequences.
仅有 3%的人类转录基因组被翻译成蛋白质，而这些非翻译区的小非编码 RNA 已被证明在蛋白质的转录和翻译调控中具有关键作用。在这里，我们提供了一个资源，将有助于选择用于涉及癌症研究中 sncRNA 的基因表达研究的细胞系。作为肿瘤最易接近和可处理的模型，癌细胞系被广泛用于研究癌症的发展和进展。NCI-60 面板包含 59 种癌细胞系，是为了在 9 种组织类型中进行药物筛选而精心挑选的；然而，它的重要性已经扩展到基因调控、异种移植模型等领域。在这里，我们展示了这 59 种癌细胞系的完整小非编码 RNA (sncRNA) 转录组。此外，我们还研究了注释 microRNA (miRNA)、PIWI 相互作用 RNA (piRNA)、小核 RNA (snRNA) 和小核仁 RNA (snoRNA) 的丰度和独特序列，并揭示了新的未注释的 microRNA 序列。
