Ensembl基因注释系统。
The Ensembl gene annotation system.
作者信息
Aken Bronwen L, Ayling Sarah, Barrell Daniel, Clarke Laura, Curwen Valery, Fairley Susan, Fernandez Banet Julio, Billis Konstantinos, García Girón Carlos, Hourlier Thibaut, Howe Kevin, Kähäri Andreas, Kokocinski Felix, Martin Fergal J, Murphy Daniel N, Nag Rishi, Ruffier Magali, Schuster Michael, Tang Y Amy, Vogel Jan-Hinnerk, White Simon, Zadissa Amonida, Flicek Paul, Searle Stephen M J
机构信息
European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK Present addresses: The Genome Analysis Centre, Norwich Research Park, Norwich NR4 7UH, UK.
出版信息
Database (Oxford). 2016 Jun 23;2016. doi: 10.1093/database/baw093. Print 2016.
The Ensembl gene annotation system has been used to annotate over 70 different vertebrate species across a wide range of genome projects. Furthermore, it generates the automatic alignment-based annotation for the human and mouse GENCODE gene sets. The system is based on the alignment of biological sequences, including cDNAs, proteins and RNA-seq reads, to the target genome in order to construct candidate transcript models. Careful assessment and filtering of these candidate transcripts ultimately leads to the final gene set, which is made available on the Ensembl website. Here, we describe the annotation process in detail.Database URL: http://www.ensembl.org/index.html.
Ensembl基因注释系统已被用于在广泛的基因组项目中注释70多种不同的脊椎动物物种。此外，它还为人类和小鼠的GENCODE基因集生成基于自动比对的注释。该系统基于生物序列（包括cDNA、蛋白质和RNA-seq读数）与目标基因组的比对，以构建候选转录本模型。对这些候选转录本进行仔细评估和筛选最终得到最终的基因集，该基因集可在Ensembl网站上获取。在这里，我们详细描述注释过程。数据库网址：http://www.ensembl.org/index.html。
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