Zhao Ying, Gao Zhixiao, Tian Binnian, Bi Kai, Chen Tao, Liu Huiquan, Xie Jiatao, Cheng Jiasen, Fu Yanping, Jiang Daohong
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei Province, P-R China.
The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province, P-R China.
PLoS One. 2017 Oct 24;12(10):e0185907. doi: 10.1371/journal.pone.0185907. eCollection 2017.
Clubroot caused by Plasmodiophora brassicae, is a severe disease of cruciferous crops that causes large hypertrophic galls in the roots. The plant microbiome is important for growth promotion and disease suppression. In this study, using 16S rRNA and internal transcribed spacer (ITS) sequencing techniques, we compared the endosphere microbiome of symptomatic and asymptomatic B. napus roots infected with P. brassicae collected from the same natural clubroot field. The results showed that the microbial population and its relative abundance in the asymptomatic roots was far higher than that in the symptomatic roots, and that many microorganisms in asymptomatic roots have biological control and plant growth promotion functions that may be related to clubroot symptoms. These results suggest the importance of the endosphere microbiome in clubroot disease and provide potential bio-control resources for its prevention.
由芸薹根肿菌引起的根肿病是十字花科作物的一种严重病害，会在根部形成巨大的肥大瘿瘤。植物微生物群对促进生长和抑制病害很重要。在本研究中，我们使用16S rRNA和内转录间隔区（ITS）测序技术，比较了从同一自然根肿病田采集的感染芸薹根肿菌的有症状和无症状甘蓝型油菜根的内生微生物群。结果表明，无症状根中的微生物种群及其相对丰度远高于有症状根，并且无症状根中的许多微生物具有生物防治和促进植物生长的功能，这可能与根肿病症状有关。这些结果表明内生微生物群在根肿病中的重要性，并为其预防提供了潜在的生物防治资源。
