1 Clinical Research Center, Schauenburgerstraβe 116, 24118 Kiel, Germany.
2 LADR, Lauenburger Str. 67, 21502 Geesthacht, Germany.
Benef Microbes. 2018 Jan 29;9(1):35-50. doi: 10.3920/BM2017.0018. Epub 2017 Oct 25.
Bacterial vaginosis (BV) is characterised by a depletion of lactobacilli in favour of an overgrowth of anaerobic bacteria. It is associated with increased risk for urogenital infections and abortion. In this study we assessed the effect of a yoghurt drink containing Lactobacillus strains on BV. The strains had been isolated from healthy pregnant women and selected for acidification capacity, production of HO, glycogen utilisation, bile salt tolerance and inhibition of pathogens. Using Amsel criteria BV was diagnosed in 36 women aged ≥18 years with stable menstrual cycle or menopause. They were treated with oral metronidazole for 7 days (2×500 mg/d). Starting with the treatment, women consumed twice daily either verum or placebo during 4 weeks. Verum was 125 g yoghurt containing (besides Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus) living strains Lactobacillus crispatus LbV 88 (DSM 22566), Lactobacillus gasseri LbV 150N (DSM 22583), Lactobacillus jensenii LbV 116 (DSM 22567) and Lactobacillus rhamnosus LbV96 (DSM 22560), each 1×107 cfu/ml; placebo was 125 g chemically acidified milk. After 4 weeks of intervention 0 of 17 had BV in the verum group versus 6 of 17 in the s.a. control (0.018 in Fisher Exact test). Amsel score decreased during the intervention period by 4.0 (median) (4.0; 3.0) (25; 75 percentile) in the verum group compared to 2.0 (4.0; 0.0) in the control group (P=0.038 in Mann-Whitney test). Discharge and odour (Amsel criteria 2+3) also decreased by 2.0 (2.0; 1.0) in the verum compared to 1.0 (2.0; 0.0) in the control group (P=0.01) and differed after 4 weeks intervention between the groups 0.0 (0.0; 0.0) versus 1.0 (0.0; 2.0) (P=0.001). Nugent score decreased during the intervention period by 5.5 (7.0;2.3) in the verum compared to 3.0 (6.0;0.5) in the control group (P=0.158). Additional intake of yoghurt containing these probiotic strains improved the recovery rate and symptoms of BV and tended to improve the vaginal microbial pattern.
细菌性阴道病（BV）的特征是乳酸杆菌减少，而厌氧菌过度生长。它与泌尿生殖系统感染和流产的风险增加有关。在这项研究中，我们评估了含有乳酸杆菌菌株的酸奶饮料对 BV 的影响。这些菌株是从健康孕妇中分离出来的，并选择了酸化能力、产生 HO、糖原利用、胆汁盐耐受性和抑制病原体的能力。使用 Amsel 标准，诊断了 36 名年龄≥18 岁、月经周期稳定或绝经的妇女患有 BV。她们接受了 7 天的口服甲硝唑治疗（2×500mg/d）。从治疗开始，女性在 4 周内每天两次分别服用真药或安慰剂。真药是含有（除了乳酸杆菌 delbrueckii ssp。保加利亚和嗜热链球菌）活菌的 125 克酸奶，含有 Lactobacillus crispatus LbV 88（DSM 22566）、Lactobacillus gasseri LbV 150N（DSM 22583）、Lactobacillus jensenii LbV 116（DSM 22567）和 Lactobacillus rhamnosus LbV96（DSM 22560），每种 1×107cfu/ml；安慰剂是 125 克化学酸化牛奶。干预 4 周后，真药组有 0/17 人患有 BV，而对照组有 6/17 人（Fisher 确切检验，0.018）。与对照组相比，真药组的 Amsel 评分在干预期间下降了 4.0（中位数）（4.0；3.0）（25；75 百分位数）（P=0.038 在 Mann-Whitney 检验中）。阴道分泌物和气味（Amsel 标准 2+3）也下降了 2.0（2.0；1.0），而对照组下降了 1.0（2.0；0.0）（P=0.01），在干预 4 周后，两组之间的差异为 0.0（0.0；0.0）与 1.0（0.0；2.0）（P=0.001）。在干预期间，真药组的 Nugent 评分下降了 5.5（7.0；2.3），而对照组下降了 3.0（6.0；0.5）（P=0.158）。额外摄入含有这些益生菌菌株的酸奶改善了 BV 的恢复率和症状，并倾向于改善阴道微生物模式。
