Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
J Ethnopharmacol. 2018 Feb 15;212:67-73. doi: 10.1016/j.jep.2017.10.017. Epub 2017 Oct 21.
Danhong Injection (DHI), a Chinese medical product extracted from Radix et Rhizoma Salviae Miltiorrhizae (Salvia miltiorrhiza Bge., Labiatae, Danshen in Chinese) and Flos Carthami (Carthamus tinctorius L., Compositae, Honghua in Chinese), has been reported to have effects on inflammatory, anti-fibrinolytic properties, antithrombotic and decrease blood-lipid. It is extensively used for the clinical treatment of cardiovascular disease. This study aimed to investigate the effects of DHI on blood-lipid levels and platelet aggregation rate in hyperlipidemia rats.
Rats were randomly divided into 6 groups: normal control (NC), model control (MC), DHI-treated control at doses of 1.0mL/kg, 2.0mL/kg, 4.0mL/kg, respectively, and Simvastatin positive control at dose of 2.0mg/kg. All DHI treated groups were intraperitoneally injected for 7 days. The effects of DHI on serum triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) were evaluated. And platelet activating factor (PAF), platelet membrane glycoprotein IIb/IIIa (GP IIb/IIIa) and 6-keto-prostaglandin F1а (6-K-PGF1а) were determined by enzyme-linked immunosorbent assay (ELISA). Moreover, the expression of prostaglandin I-2 (PGI), prostaglandin E-2 (PGE) and thromboxane A2 (TXA) in liver was determined by real-time PCR.
Compared with the MC group, the rats treated with DHI had significantly reduced TC, TG, LDL-C, FIB, GP IIb/IIIa and platelet aggregation. Meanwhile, the thrombin time (TT), activated partial thrombin time (APTT), prothrombin time (PT), 6-K-PGF1а was significantly increased. Expression of PGI and PGE mRNA was significantly increased, whereas the TXA was significantly reduced.
This study demonstrated that the blood lipid and platelet aggregation has a regulatory effect after DHI treatment. The insights gained from this study will improve understanding of the mechanisms involved in the effect of DHI on hyperlipidemia and the pharmacological rationale for the use of DHI in diseases caused by formation of thrombosis and lipid metabolic disorders.
丹红注射液（DHI）是一种从丹参（丹参，唇形科，丹参）和红花（红花，菊科，红花）中提取的中药制剂，已被报道具有抗炎、抗纤溶、抗血栓和降血脂作用。它被广泛用于心血管疾病的临床治疗。本研究旨在探讨 DHI 对高血脂大鼠血脂水平和血小板聚集率的影响。
大鼠随机分为 6 组：正常对照组（NC）、模型对照组（MC）、DHI 治疗组（剂量分别为 1.0mL/kg、2.0mL/kg、4.0mL/kg）和辛伐他汀阳性对照组（剂量为 2.0mg/kg）。所有 DHI 治疗组均连续 7 天腹腔注射。评估 DHI 对血清甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C）的影响。并通过酶联免疫吸附试验（ELISA）测定血小板激活因子（PAF）、血小板膜糖蛋白 IIb/IIIa（GP IIb/IIIa）和 6-酮-前列腺素 F1a（6-K-PGF1a）。此外，通过实时 PCR 测定肝组织中前列腺素 I-2（PGI）、前列腺素 E-2（PGE）和血栓烷 A2（TXA）的表达。
与 MC 组相比，DHI 治疗组 TC、TG、LDL-C、纤维蛋白原、GP IIb/IIIa 和血小板聚集明显降低。同时，凝血酶时间（TT）、活化部分凝血活酶时间（APTT）、凝血酶原时间（PT）、6-K-PGF1a 明显升高。PGI 和 PGE mRNA 的表达明显增加，而 TXA 明显减少。
本研究表明，DHI 治疗后对血脂和血小板聚集有调节作用。本研究的结果将提高对 DHI 对高血脂影响的作用机制的认识，并为 DHI 在由血栓形成和脂质代谢紊乱引起的疾病中的应用提供药理学依据。
