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降钙素基因相关肽修饰的Bio-Oss促进骨生成。

Bio-Oss modified by calcitonin gene-related peptide promotes osteogenesis .

作者信息

Li Yuanjing, Yang Lan, Zheng Zhichao, Li Zhengmao, Deng Tian, Ren Wen, Wu Caijuan, Guo Lvhua

机构信息

Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China.

出版信息

Exp Ther Med. 2017 Nov;14(5):4001-4008. doi: 10.3892/etm.2017.5048. Epub 2017 Aug 28.

DOI:10.3892/etm.2017.5048
PMID:29067095
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5647716/
Abstract

Bio-Oss and α-calcitonin gene-related peptide (CGRP) are involved in osteogenesis. However, it has remained to be assessed how α-CGRP affects the effect of Bio-Oss. In the present study, primary osteoblasts were incubated with α-CGRP, Bio-Oss, α-GGRP-Bio-Oss or mimic-α-CGRP. The proliferation rate, mineralization nodules, alkaline phosphatase (ALP) activity and the expression of osteogenic genes were measured by a Cell Counting Kit-8 assay, Alizarin Red-S staining, ALP activity detection and reverse-transcription quantitative PCR as well as western blot analysis, respectively. The proliferation rate, ALP activity and the number of mineralization nodules were significantly increased in the α-CGRP-modified Bio-Oss group compared to that in the Bio-Oss group. The mRNA and protein levels of osteocalcin, Runt-related transcription factor-2 and ALP were significantly upregulated in the α-CGRP-Bio-Oss group compared with those in the Bio-Oss group. Furthermore, the effect of mimic-α-CGRP on osteogenesis was reduced as it carried a mutation. In conclusion, the present study was the first to demonstrate that Bio-Oss modified with CGRP contributed to osteogenesis and may provide a novel formulation applied in the clinic for restoration of large bone defects.

摘要

骨粉（Bio-Oss）和α-降钙素基因相关肽（CGRP）参与骨生成。然而，α-CGRP如何影响骨粉的作用仍有待评估。在本研究中，将原代成骨细胞与α-CGRP、骨粉、α-CGRP-骨粉或模拟α-CGRP一起孵育。分别通过细胞计数试剂盒-8检测、茜素红S染色、碱性磷酸酶（ALP）活性检测以及逆转录定量PCR和蛋白质印迹分析来测量增殖率、矿化结节、ALP活性和成骨基因的表达。与骨粉组相比，α-CGRP修饰的骨粉组的增殖率、ALP活性和矿化结节数量显著增加。与骨粉组相比，α-CGRP-骨粉组中骨钙素、Runt相关转录因子2和ALP的mRNA和蛋白质水平显著上调。此外，模拟α-CGRP携带突变后对骨生成的作用减弱。总之，本研究首次证明用CGRP修饰的骨粉有助于骨生成，并可能为临床应用提供一种用于修复大骨缺损的新型制剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/5c3957ced3e4/etm-14-05-4001-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/a931e0b9e5ed/etm-14-05-4001-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/6f2af378cca6/etm-14-05-4001-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/bf2232001e96/etm-14-05-4001-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/970e6d5eb113/etm-14-05-4001-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/810547ebdb06/etm-14-05-4001-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/ec7f8f179443/etm-14-05-4001-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/e96424e4b3b5/etm-14-05-4001-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/5c3957ced3e4/etm-14-05-4001-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/a931e0b9e5ed/etm-14-05-4001-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/6f2af378cca6/etm-14-05-4001-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/bf2232001e96/etm-14-05-4001-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/970e6d5eb113/etm-14-05-4001-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/810547ebdb06/etm-14-05-4001-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/ec7f8f179443/etm-14-05-4001-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/e96424e4b3b5/etm-14-05-4001-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cf2/5647716/5c3957ced3e4/etm-14-05-4001-g07.jpg

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