Kasuya Kazuhiko, Oka Kiyoshi, Soya Ryoko, Tsuchiya Takaaki, Itoi Takao, Tsuchida Akihiko
Department of Gastrointestinal and Pediatric Surgery, Tokyo Medical University, Tokyo 160-0023, Japan.
OK Fiber Technology Co., Ltd., Kyoto 619-0215, Japan.
Exp Ther Med. 2017 Nov;14(5):4344-4348. doi: 10.3892/etm.2017.5052. Epub 2017 Aug 28.
Photodynamic therapy (PDT) is a method used treat tumors via utilizing photodynamic reactions between photosensitive substances with tumor affinity and lasers. For overall bile duct cancers, PDT has been demonstrated to resolve stenosis and improve prognosis; however, when limited to intrahepatic bile duct cancers, modifications to the laser irradiation are necessary as surrounding hepatocytes incorporate a large amount of photosensitive substances. Furthermore, the intrahepatic bile duct is thin, and a guide sheath and thin fiber are necessary to transport laser irradiation probes to the target region. In the present study, a parallel-type ultra-small composite optical fiberscope (COF) with an outer diameter of 1 mm or smaller was developed to target a thin intrahepatic bile duct. PDT was performed using an animal model and talaporfin sodium (Laserphyrin), which is rapidly excreted by hepatocytes and is suitable for use with a long-wavelength laser due to its high tissue penetrating ability. The results demonstrated that Laserphyrin does not cause necrotic changes in the normal biliary tract mucosa. In addition, COF images of sufficient quality were acquired. The present results suggest that COF may be used for the treatment of deep bile duct lesions.
光动力疗法（PDT）是一种通过利用具有肿瘤亲和力的光敏物质与激光之间的光动力反应来治疗肿瘤的方法。对于总体胆管癌，已证明PDT可解决狭窄并改善预后；然而，对于肝内胆管癌，由于周围肝细胞会摄取大量光敏物质，因此需要对激光照射进行调整。此外，肝内胆管较细，需要使用引导鞘和细光纤将激光照射探头输送到目标区域。在本研究中，开发了一种外径为1毫米或更小的平行型超小型复合光纤镜（COF），用于靶向肝内细胆管。使用动物模型和他拉泊芬钠（Laserphyrin）进行PDT，他拉泊芬钠可被肝细胞快速排泄，由于其高组织穿透能力，适用于长波长激光。结果表明，Laserphyrin不会引起正常胆道黏膜的坏死变化。此外，还获得了质量足够的COF图像。目前的结果表明，COF可用于治疗深部胆管病变。
