Stefan-van Staden Raluca-Ioana, Balahura Liliana-Roxana, Gugoasa Livia Alexandra, van Staden Jacobus F, Aboul-Enein Hassan Y, Rosu Marcela-Corina, Pruneanu Stela Maria
Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest-6, Romania.
The Pharmaceutical and Drug Industries Research Division, Pharmaceutical and Medicinal Chemistry Department, National Research Centre, Dokki, Cairo, 12311, Egypt.
Anal Bioanal Chem. 2018 Jan;410(1):115-121. doi: 10.1007/s00216-017-0698-7. Epub 2017 Oct 24.
8-Hydroxy-2'-deoxyguanosine (8-OHdG), a product of oxidative DNA damage, which has been used as a sensitive and reliable marker of oxidative stress and carcinogenesis, is found in high levels in biological fluids of leukemia patients. A reliable screening method based on pattern recognition of 8-OHdG using stochastic sensors designed with graphene materials decorated with nanoparticles of TiOAg or TiOAu was developed. 5,10,15,20-Tetraphenyl-21H,23H porphyrin (P), 2,6-bis((E)-2-(furan-2-yl)vinyl)-4-(4,6,8-trimethylazulen-1-yl)pyridine (Py1), and 2,6-bis((E)-2-(thiophen-3-yl)vinyl)-4-(4,6,8-trimethylazulen-1-yl)pyridine (Py2) were used as modifiers of the graphene paste in the design of sensors. The screening method used for pattern recognition was developed for two pH values accordingly with the nature of the biological fluid to be screened: pH = 3.02 for urine samples and pH = 7.49 for whole blood samples. High sensitivities and low limits of determination for 8-OHdG obtained at both pH values favored the early detection of leukemia. Recoveries over 98.00% with RSD (%) values lower than 1.00% proved the reliability of the proposed screening method. Graphical abstract Graphene based sensors detect 8-hydroxy-2'-deoxyguanosine in biological fluids.
8-羟基-2'-脱氧鸟苷（8-OHdG）是氧化DNA损伤的产物，已被用作氧化应激和致癌作用的敏感且可靠的标志物，在白血病患者的生物体液中含量很高。基于使用用TiOAg或TiOAu纳米颗粒修饰的石墨烯材料设计的随机传感器对8-OHdG进行模式识别，开发了一种可靠的筛选方法。在传感器设计中，5,10,15,20-四苯基-21H，23H卟啉（P）、2,6-双（（E）-2-（呋喃-2-基）乙烯基）-4-（4,6,8-三甲基薁-1-基）吡啶（Py1）和2,6-双（（E）-2-（噻吩-3-基）乙烯基）-4-（4,6,8-三甲基薁-1-基）吡啶（Py2）被用作石墨烯糊剂的改性剂。根据待筛选生物体液的性质，针对两个pH值开发了用于模式识别的筛选方法：尿液样本为pH = 3.02，全血样本为pH = 7.49。在这两个pH值下获得的8-OHdG的高灵敏度和低检测限有利于白血病的早期检测。回收率超过98.00%，相对标准偏差（RSD）（%）值低于1.00%，证明了所提出筛选方法的可靠性。图形摘要基于石墨烯的传感器检测生物体液中的8-羟基-2'-脱氧鸟苷。
