Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest-6, Romania.
Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest-6, Romania.
Anal Biochem. 2020 Sep 15;605:113839. doi: 10.1016/j.ab.2020.113839. Epub 2020 Jul 20.
Three stochastic microsensors based on graphite powder modified with three different oleamides: N-(2-piperidin-1-ylethyl)oleamide, N-(3,4-dihydroxyphenethyl)oleamide and N-(2-morpholinoethyl)oleamide, were designed, characterized, and used to assess DNA damage in cancer by assaying two biomarkers namely 8-nitroguanine and 8-hydroxy-2'-deoxyguanosine. The two biomarkers were determined from urine and whole blood samples. The characterization of the microsensors was done at two pHs 7.40 and 3.00. The best microsensor for the simultaneous determination of biomarkers in whole blood and urine samples was the one based on the graphite paste modified with N-(3,4-dihydroxyphenethyl)oleamide. The results indicated that the proposed microsensors can be reliably used for pattern recognition and quantitative determination of 8-nitroguanine and 8-hydroxy-2'-deoxyguanosine in whole blood and urine, and accordingly, for the assessment of DNA damage in cancer patients.
基于三种不同的油酰胺（N-(2-哌啶-1-基)油酰胺、N-(3,4-二羟基苯乙基)油酰胺和 N-(2-吗啉代乙基)油酰胺）修饰石墨粉的三种随机微传感器被设计、表征，并用于通过测定两种生物标志物 8-硝基鸟嘌呤和 8-羟基-2'-脱氧鸟苷来评估癌症中的 DNA 损伤。这两种生物标志物是从尿液和全血样本中检测出来的。微传感器的特性在两个 pH 值 7.40 和 3.00 下进行了测定。用于同时测定全血和尿液样本中生物标志物的最佳微传感器是基于 N-(3,4-二羟基苯乙基)油酰胺修饰的石墨糊微传感器。结果表明，所提出的微传感器可用于可靠地对全血和尿液中的 8-硝基鸟嘌呤和 8-羟基-2'-脱氧鸟苷进行模式识别和定量测定，并可用于评估癌症患者的 DNA 损伤。
