Jin Decai, Kong Xiao, Jia Minghong, Yu Xiangyang, Wang Xinxin, Zhuang Xuliang, Deng Ye, Bai Zhihui
CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.
College of Resources and Environment, University of the Chinese Academy of Sciences, Beijing 100049, PR China.
Int J Syst Evol Microbiol. 2017 Dec;67(12):5128-5133. doi: 10.1099/ijsem.0.002430. Epub 2017 Oct 25.
A phthalate esters-degrading bacterial strain, designated QH-11, was isolated from an activated sludge wastewater treatment plant in Beijing, PR China. The cells were aerobic, Gram-stain-positive, non-motile, catalase-positive, oxidase-negative, short rods and formed white colonies on trypticase soy agar. This isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and whole-cell hydrolysates contained arabinose and ribose. Diphosphatidylglycerol and phosphatidylethanolamine were the predominant polar lipids. According to the results of full-length of 16S rRNA gene sequence analysis, QH-11 represented a member of the genus Gordonia and showed the highest sequence similarity to Gordonia hydrophobica DSM 44015 (99.2 %), but was distinguishable by a low level of DNA-DNA relatedness (37.8 %). Genome-based comparisons indicated a clear distinction from the top ten most similar type strains (16S rRNA gene sequence) with pairwise average nucleotide identities (ANI) between 74.6 and 83.4 %. The predominant respiratory quinone was MK-9(H2), the mycolic acids present had 56 to 62 carbon atoms, and the major fatty acids were C16 : 0 (33.3 %), C17 : 1ω8c (23.4 %) and C18 : 1ω9c (17.9 %). The DNA G+C content was 68.0 mol%. On the basis of the results of DNA-DNA hybridization, ANI and physiological and biochemical tests, it is proposed that QH-11 represents a novel species of the genus Gordonia, for which the name Gordonia phthalatica sp. nov. is proposed. The type strain is QH-11 (CICC 24107 =KCTC 39933).
从中国北京的一座活性污泥废水处理厂中分离出一株可降解邻苯二甲酸酯的细菌菌株，命名为QH-11。该菌为需氧菌，革兰氏染色阳性，无运动性，过氧化氢酶阳性，氧化酶阴性，呈短杆状，在胰蛋白胨大豆琼脂上形成白色菌落。该分离株含有内消旋二氨基庚二酸作为诊断性二氨基酸，全细胞水解产物含有阿拉伯糖和核糖。双磷脂酰甘油和磷脂酰乙醇胺是主要的极性脂质。根据16S rRNA基因序列全长分析结果，QH-11属于戈登氏菌属，与嗜疏水戈登氏菌DSM 44015的序列相似性最高（99.2%），但通过低水平的DNA-DNA相关性（37.8%）可加以区分。基于基因组的比较表明，它与前十种最相似的模式菌株（16S rRNA基因序列）有明显区别，两两平均核苷酸同一性（ANI）在74.6%至83.4%之间。主要呼吸醌为MK-9(H2)，存在的分枝菌酸含有56至62个碳原子，主要脂肪酸为C16 : 0（33.3%）、C17 : 1ω8c（23.4%）和C18 : 1ω9c（17.9%）。DNA G+C含量为68.0 mol%。基于DNA-DNA杂交、ANI以及生理生化测试结果，建议将QH-11认定为戈登氏菌属的一个新物种，命名为邻苯二甲酸戈登氏菌（Gordonia phthalatica sp. nov.）。模式菌株为QH-11（CICC 24107 =KCTC 39933）。
