Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, 99, Daehak-ro, Yuseong, Daejeon 34134, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Mar;70(3):2079-2083. doi: 10.1099/ijsem.0.004023.
A mycolic acid-containing actinobacterium designated strain MMS17-SY073 was isolated from island soil. The isolate showed best growth at 25 °C, pH 6, and 0 % (w/v) NaCl. The phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MMS17-SY073 belongs to the genus , and is mostly related to the type strains of (98.5 % sequence similarity), (98.1%), and (97.8%). The genome-based comparisons showed a clear distinction between the strain and the two neighbouring species, and , with the average nucleotide identities (ANI) of 75.8 and 76.3 %, respectively. Notably, the genome of strain MMS17-SY073 was the largest in total stretch and gene counts among the complete genomes of , and contained a number of biosynthetic gene clusters for secondary metabolites, in particular those for non-ribosomal peptide synthetases. The major polar lipids were diphosphatidyl glycerol (DPG), phosphatidyl glycerol (PG), phosphatidyl ethanolamine (PE), phosphatidyl inositol (PI) and phosphatidyl inositol mannoside (PIM). The isoprenoid quinone was MK-9(H), and the main fatty acids were C (30.2%) and 10-methyl-C (33.7%). The whole cell hydrolysates contained galactose, arabinose, and -diaminopimelic acid. The DNA G+C content was 67.4 mol%. Based on phenotypic, chemotaxonomic and genetic analysis, strain MMS17-SY073 should be classified as a new species of the genus , for which the name sp. nov. is proposed (type strain=MMS17-SY073=KCTC 49257=JCM 33277).
一株含 Mycolic 酸放线菌，命名为 MMS17-SY073，从海岛土壤中分离得到。该菌株在 25°C、pH6 和 0%（w/v）NaCl 下生长最好。基于 16S rRNA 基因序列的系统发育分析表明，菌株 MMS17-SY073 属于 属，与 （98.5%序列相似性）、 （98.1%）和 （97.8%）的模式菌株最为相似。基于基因组的比较表明，该菌株与两个相邻物种 和 之间有明显的区别，其平均核苷酸同一性（ANI）分别为 75.8%和 76.3%。值得注意的是，菌株 MMS17-SY073 的基因组在总长度和基因数量方面均为 属中最大的，并且包含了许多次级代谢产物生物合成基因簇，特别是非核糖体肽合成酶。主要极性脂为双磷脂酰甘油（DPG）、磷脂酰甘油（PG）、磷脂酰乙醇胺（PE）、磷脂酰肌醇（PI）和磷脂酰肌醇甘露糖苷（PIM）。异戊烯醌为 MK-9（H），主要脂肪酸为 C（30.2%）和 10-甲基-C（33.7%）。全细胞水解产物含有半乳糖、阿拉伯糖和 -二氨基庚二酸。DNA G+C 含量为 67.4mol%。基于表型、化学分类和遗传分析，菌株 MMS17-SY073 应归类为 属的一个新种，命名为 sp. nov.（模式菌株=MMS17-SY073=KCTC 49257=JCM 33277）。
