College of Tropical Crops, Hainan University, Haikou 570228, PR China.
Key Laboratory of Tropical Biological Resources of the Ministry of Education of China, Hainan University, Haikou 570228, PR China.
Int J Syst Evol Microbiol. 2020 Aug;70(8):4537-4543. doi: 10.1099/ijsem.0.004310. Epub 2020 Jul 15.
A novel actinobacterium, designated strain HNM0687, was isolated from mangrove soil samples collected from Hainan Province, PR China and its polyphasic taxonomy was studied. Based on the results of 16S rRNA gene sequence analysis, strain HNM0687 was closely related to NBRC 16047 (98.7 %), NBRC 16068 (98.2 %), RS15-1S (97.9 %), NBRC 16320 (97.7 %) and AMA 120 (97.7 %). Genome-based comparisons revealed a clear distinction in average nucleotide identity values between strain HNM0687 and its closely related strains (74.4-78.3 %). Strain HNM0687 contained -diaminopimelic acid, arabinose and galactose in whole-cell hydrolysates. Mycolic acid was present. The menaquinones of strain HNM0687 were MK-9(H) and MK-7(H). The phospholipids of the isolate were composed of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were C, C 7/C 6, C10-methyl (TBSA), C and C 9. Based on its genotypic, chemotaxonomic and phenotypic characteristics, it is concluded that strain HNM0687 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is HNM0687 (=CCTCC AA 2019074 =KCTC 49383 ).
一株新的放线菌，命名为 HNM0687 菌株，从中国海南省红树林土壤样本中分离得到，对其进行了多相分类学研究。基于 16S rRNA 基因序列分析的结果，菌株 HNM0687 与 NBRC 16047（98.7%）、NBRC 16068（98.2%）、RS15-1S（97.9%）、NBRC 16320（97.7%）和 AMA 120（97.7%）最为接近。基于基因组的比较表明，菌株 HNM0687 与密切相关的菌株之间的平均核苷酸同一性值有明显差异（74.4-78.3%）。菌株 HNM0687 含有 -二氨基庚二酸、阿拉伯糖和半乳糖。存在分枝菌酸。菌株 HNM0687 的menaquinones 为 MK-9(H) 和 MK-7(H)。分离物的磷脂由双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰肌醇组成。主要脂肪酸为 C 、C 7/C 6 、C10-methyl（TBSA）、C 和 C 9 。基于其基因型、化学分类学和表型特征，推断菌株 HNM0687 代表一个新的属种，建议命名为 sp. nov.。该模式菌株为 HNM0687（=CCTCC AA 2019074 =KCTC 49383 ）。
