Liu Tianhao, Yang Zhongshan, Zhang Xiaomei, Han Niping, Yuan Jiali, Cheng Yu
School of Basic Medical Sciences, Yunnan University of Traditional Chinese Medicine, Yuhua Road, No. 1024, Kunming, 650000 Yunnan People's Republic of China.
Graduate School, Yunnan University of Traditional Chinese Medicine, Yuhua Road, No. 1024, Kunming, 650000 Yunnan People's Republic of China.
3 Biotech. 2017 Dec;7(6):370. doi: 10.1007/s13205-017-0997-x. Epub 2017 Oct 12.
This study aims to explore the effect of FMT on regulations of dysbacteriosis of pulmonary and intestinal flora in rats with 16S rDNA sequencing technology. A total of 27 SPF rats (3-4 weeks old) were randomly divided into three groups: normal control group (K), model control group (MX), and fecal microbiota transplantation group (FMT); each group contained nine rats. The OTU values of the pulmonary and intestinal flora of the MX group decreased significantly compared with the normal control group. After FMT, the OTU value of pulmonary flora increased, while the value of OTU in intestinal flora declined. At the phylum level, FMT down-regulated , , and in the pulmonary flora. At the genus level, FMT down-regulated , , , , and , thus maintaining the balance of the pulmonary flora. Moreover, FMT could change the structure and diversity of the pulmonary and intestinal flora by positively regulating the pulmonary flora and negatively regulating intestinal flora. This study may provide a scientific basis for FMT treatment of respiratory diseases.
本研究旨在运用16S rDNA测序技术探讨粪菌移植（FMT）对大鼠肺和肠道菌群失调的调节作用。将27只3 - 4周龄的SPF大鼠随机分为三组：正常对照组（K）、模型对照组（MX）和粪菌移植组（FMT）；每组9只大鼠。与正常对照组相比，MX组肺和肠道菌群的OTU值显著降低。FMT后，肺菌群的OTU值升高，而肠道菌群的OTU值下降。在门水平上，FMT下调了肺菌群中的 、 和 。在属水平上，FMT下调了 、 、 、 和 ，从而维持肺菌群的平衡。此外，FMT可通过正向调节肺菌群和负向调节肠道菌群来改变肺和肠道菌群的结构与多样性。本研究可能为FMT治疗呼吸系统疾病提供科学依据。
