Chen Rongrong, Wu Fangmei, Zeng Guannan, Chen Yuanchun, Lu Shiyun, Huang Huping
Provincial Clinical Medical College of Fujian Medical University, Fuzhou 350001, Fujian, China.
Department of Gastroenterology, Fujian Provincial Hospital South Branch, Fuzhou 350028, Fujian, China.
Iran J Basic Med Sci. 2024;27(9):1148-1154. doi: 10.22038/IJBMS.2024.74280.16142.
This study aimed to explore the therapeutic effect of fecal microbiota transplantation (FMT) on liver cirrhosis-induced rat models by studying changes in intestinal flora distribution and liver pathology.
Cirrhosis was induced in adult male Sprague-Dawley rats using carbon tetrachloride; successful establishment of the cirrhosis model was verified using hematoxylin and eosin (HE) staining. Rats were divided into normal control, cirrhosis model+normal saline, and cirrhosis model+FMT groups. Fecal intestinal flora was analyzed using 16S rRNA high-throughput sequencing for each group. Alpha diversity, beta diversity, and functional prediction analyses were performed. Additionally, rat liver tissue was subjected to HE staining to compare the degree of fibrosis and liver damage between the groups.
FMT significantly improved the diversity, richness, and uniformity of the intestinal flora in rats with liver cirrhosis. Notably, post-FMT, the abundance of lactobacillaceae, bacilli, and bacteroidia increased, while the abundance of clostridia decreased. Moreover, hepatic fibrosis improved after FMT.
The dysbiosis of intestinal flora in rats with liver cirrhosis improved after FMT. Thus, FMT can regulate intestinal flora, reduce liver inflammation, and improve hepatic fibrosis and cirrhosis.
本研究旨在通过研究肠道菌群分布变化和肝脏病理学，探讨粪便微生物群移植（FMT）对肝硬化诱导大鼠模型的治疗效果。
使用四氯化碳诱导成年雄性Sprague-Dawley大鼠肝硬化；用苏木精-伊红（HE）染色验证肝硬化模型的成功建立。将大鼠分为正常对照组、肝硬化模型+生理盐水组和肝硬化模型+FMT组。对每组大鼠的粪便肠道菌群进行16S rRNA高通量测序分析。进行α多样性、β多样性和功能预测分析。此外，对大鼠肝脏组织进行HE染色，比较各组之间的纤维化程度和肝损伤情况。
FMT显著改善了肝硬化大鼠肠道菌群的多样性、丰富度和均匀性。值得注意的是，FMT后，乳杆菌科、芽孢杆菌和拟杆菌的丰度增加，而梭菌的丰度降低。此外，FMT后肝纤维化得到改善。
FMT后肝硬化大鼠肠道菌群失调得到改善。因此，FMT可以调节肠道菌群，减轻肝脏炎症，改善肝纤维化和肝硬化。
