Esmaeillou Mahsa, Zarrini Gholamreza, Ahangarzadeh Rezaee Mohammad, Shahbazi Mojarrad Javid, Bahadori Ali
Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Adv Pharm Bull. 2017 Sep;7(3):479-483. doi: 10.15171/apb.2017.058. Epub 2017 Sep 25.
Many antimicrobial medications are available to combat infections. However, the indiscriminate use of antibiotics has produced antibiotic resistance in the case of many bacterial pathogens. This study focuses on the development of nanoparticles (NPs) that enhance the in vitro antibiotic activity of vancomycin against multi-drug resistant (MDR) organisms. Spherical shaped thioglycolic acid-stabilized silver nanoparticles (TGA-AgNPs) were prepared by using a simple chemical reduction method. Then, vancomycin was conjugated to the terminal carboxyl of TGA in the presence of N-Hydroxysuccinimide (NHS) and N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC). Afterwards, the antibacterial activity of these nanoconjugates was examined by using the minimum inhibitory concentration (MIC) assay against MDR bacteria. The rate of vancomycin bound to the AgNPs was 19.6%. The MIC values of vancomycin (Van)-capped AgNPs against tested pathogens were in the range of (3.2, 1.6, 0.8, 0.4, 0.2, 0.1, 0.05, and 0.025 µl/ml). The MIC was 0.1 µg/ml for VRE, MIC≤0.02 µg/ml for MRSE, and 0.05 µg/ml for S. aureus. The MIC corresponded to the MBC for all bacterial species. This study indicated that some antimicrobial agents like vancomycin can be conjugated with AgNPs. This can lead to increased antimicrobial activity against MDR microorganisms.
有许多抗菌药物可用于对抗感染。然而，抗生素的滥用已导致许多细菌病原体产生抗生素耐药性。本研究聚焦于纳米颗粒（NPs）的研发，这类纳米颗粒可增强万古霉素对多重耐药（MDR）微生物的体外抗菌活性。通过简单的化学还原法制备了球形巯基乙酸稳定的银纳米颗粒（TGA-AgNPs）。然后，在N-羟基琥珀酰亚胺（NHS）和N-(3-二甲基氨基丙基)-N'-乙基碳二亚胺盐酸盐（EDC）存在的情况下，将万古霉素与TGA的末端羧基偶联。之后，通过针对MDR细菌的最低抑菌浓度（MIC）测定法检测这些纳米偶联物的抗菌活性。万古霉素与AgNPs的结合率为19.6%。万古霉素包被的AgNPs对受试病原体的MIC值范围为（3.2、1.6、0.8、0.4、0.2、0.1、0.05和0.025 µl/ml）。耐万古霉素肠球菌（VRE）的MIC为0.1 µg/ml，耐甲氧西林表皮葡萄球菌（MRSE）的MIC≤0.02 µg/ml，金黄色葡萄球菌的MIC为0.05 µg/ml。对于所有细菌种类，MIC均对应于最低杀菌浓度（MBC）。本研究表明，一些抗菌剂如万古霉素可与AgNPs偶联。这可导致对MDR微生物的抗菌活性增强。
