3-氨丙基三乙氧基硅烷包覆的氧化铁纳米颗粒对枯草芽孢杆菌生产甲萘醌-7的影响。

Ranmadugala Dinali, Ebrahiminezhad Alireza, Manley-Harris Merilyn, Ghasemi Younes, Berenjian Aydin

Faculty of Science and Engineering, University of Waikato, Hamilton 3216, New Zealand.

Department of Medical Biotechnology, School of Medicine and Noncommunicable Diseases Research Centre, Fasa University of Medical Sciences, Fasa 74615, Iran.

Nanomaterials (Basel). 2017 Oct 26;7(11):350. doi: 10.3390/nano7110350.

One of the major issues associated with industrial production of menaquinone-7 (MK-7) is the low fermentation yield. In this study, we investigated the effect of iron oxide nanoparticles coated with 3-aminopropyltriethoxysilane (IONs@APTES) on the production of MK-7 using (ATCC 6633). Decoration of cells with IONs@APTES significantly enhanced both MK-7 production and yield. An approximately two-fold increase in MK-7 production (41 mg/L) was observed in the presence of 500 µg/mL IONs@APTES, as compared to MK-7 production using untreated bacteria (22 mg/L). This paper, therefore, illustrates the immense biotechnological potential of IONs@APTES in increasing MK-7 concentration using , and its future role in bioprocess engineering.

与甲萘醌-7（MK-7）工业生产相关的主要问题之一是发酵产量低。在本研究中，我们研究了用3-氨丙基三乙氧基硅烷包覆的氧化铁纳米颗粒（IONs@APTES）对使用（ATCC 6633）生产MK-7的影响。用IONs@APTES修饰细胞显著提高了MK-7的产量和产率。与使用未处理细菌生产的MK-7（22 mg/L）相比，在存在500 µg/mL IONs@APTES的情况下，MK-7产量增加了约两倍（41 mg/L）。因此，本文阐述了IONs@APTES在利用增加MK-7浓度方面的巨大生物技术潜力及其在生物过程工程中的未来作用。