Ebrahiminezhad Alireza, Varma Vikas, Yang Shuyi, Ghasemi Younes, Berenjian Aydin
Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa 74615, Iran.
Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz 71348, Iran.
Nanomaterials (Basel). 2015 Dec 25;6(1):1. doi: 10.3390/nano6010001.
Industrial production of menaquione-7 by is associated with major drawbacks. To address the current challenges in menaquione-7 fermentation, studying the effect of magnetic nanoparticles on the bacterial cells can open up a new domain for intensified menqainone-7 process. This article introduces the new concept of production and application of l-lysine coated iron oxide nanoparticles (l-Lys@IONs) as a novel tool for menaquinone-7 biosynthesis. l-Lys@IONs with the average size of 7 nm were successfully fabricated and were examined in a fermentation process of l-Lys@IONs decorated . Based on the results, higher menaquinone-7 specific yield was observed for l-Lys@IONs decorated bacterial cells as compared to untreated bacteria. In addition, more than 92% removal efficacy was achieved by using integrated magnetic separation process. The present study demonstrates that l-Lys@IONs can be successfully applied during a fermentation of menaquinone-7 without any negative consequences on the culture conditions. This study provides a novel biotechnological application for IONs and their future role in bioprocess intensification.
通过[具体方式]进行维生素K7的工业化生产存在重大缺陷。为应对当前维生素K7发酵面临的挑战，研究磁性纳米颗粒对细菌细胞的影响可为强化维生素K7生产工艺开辟新领域。本文介绍了用L-赖氨酸包覆的氧化铁纳米颗粒（l-Lys@IONs）作为维生素K7生物合成新工具的生产及应用的新概念。成功制备了平均尺寸为7纳米的l-Lys@IONs，并在l-Lys@IONs修饰的[具体对象]发酵过程中对其进行了检测。结果表明，与未处理的细菌相比，l-Lys@IONs修饰的细菌细胞的维生素K7比产量更高。此外，采用集成磁分离工艺可实现92%以上的去除效率。本研究表明，l-Lys@IONs可成功应用于维生素K7的发酵过程，且对培养条件无任何负面影响。该研究为离子纳米颗粒提供了一种新的生物技术应用及其在生物过程强化中的未来作用。
