Yan Yan, Zhang Shuang, Tao Guan-Jun, You Feng-Hui, Chen Jie, Zeng Mao-Mao
Institute of Agro-products Processing, Anhui Academy of Agricultural Science, Hefei 230031, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Nov 15;1068-1069:173-179. doi: 10.1016/j.jchromb.2017.10.015. Epub 2017 Oct 9.
This study proposed a simple and accurate acetonitrile extraction pretreatment method coupled with ultrahigh-performance liquid chromatography with tandem mass spectrometry for the simultaneous determination of 17 heterocyclic aromatic amines (HAAs) in meat products. With this new method, all 17 HAAs, including 11 polar and 6 nonpolarHAAs, were simultaneously extracted by acetonitrile and purified by one-step Oasis MCX cartridge purification. Compared with two different improved reference methods, the acetonitrile method could obtain higher recoveries (in the range of 42.5% to 99.0%) and better repeatability (lower than 12.2%). The limits of quantification were calculated between 0.028nggand0.648ngg with high correlation coefficients (r>0.9976) in wide linear ranges. The proposed acetonitrile method was successfully applied to the analysis of the HAAs levels in 10 commercial meat products with satisfactory recoveries.
本研究提出了一种简单准确的乙腈萃取预处理方法，结合超高效液相色谱 - 串联质谱法，用于同时测定肉制品中的17种杂环芳香胺（HAA）。采用这种新方法，17种HAA（包括11种极性和6种非极性HAA）均通过乙腈同时萃取，并通过一步Oasis MCX柱净化。与两种不同的改进参考方法相比，乙腈法可获得更高的回收率（42.5%至99.0%）和更好的重复性（低于12.2%）。在较宽的线性范围内，定量限在0.028ng/g至0.648ng/g之间，相关系数较高（r>0.9976）。所提出的乙腈法成功应用于10种市售肉制品中HAA水平的分析，回收率令人满意。
