National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
Biosens Bioelectron. 2018 Mar 15;101:174-180. doi: 10.1016/j.bios.2017.10.037. Epub 2017 Oct 18.
In this paper, novel heparin-mimicking hyperbranched polyester nanoparticles (HBPE-SO NPs) with abundant of sulfonated acid functional groups were synthesized, and their antithrombogenicities were further evaluated. Further, a label-free electrochemical aptamer biosensor (aptasensor) based on HBPE-SO NPs modified electrode was developed for thrombin (TB) detection in whole blood. Meanwhile, the anti-biofouling properties of different modified electrodes were studied by whole blood and platelet adhesion test, hemolysis assay and morphological changes of red blood cells in vitro. Besides, the thrombin-binding aptamer was selected as receptor for the proposed aptasensor, which has excellent binding affinity and selectivity for TB. When binding to TB, the electron transfer taking place at the modified electrode interface was inhibited that can attribute to the stereo-hindrance effect, resulting in the decreased current response. This aptasensor showed excellent electrochemical properties with a wide detection range and a low detection limit of 0.031pM (S/N = 3), and provided high selectivity, long-term stability and good reproducibility. Finally, the sensitively detection of TB in whole blood samples directly was achieved by this aptasensor we proposed, which suggested its great potential for TB detection in the clinic.
本文合成了具有丰富磺酸基官能团的新型肝素模拟超支化聚酯纳米粒子（HBPE-SO NPs），并进一步评价了其抗血栓形成性能。进一步，基于 HBPE-SO NPs 修饰电极构建了一种无标记的电化学适体传感器（aptasensor），用于在全血中检测凝血酶（TB）。同时，通过全血和血小板黏附试验、溶血试验和体外红细胞形态变化研究了不同修饰电极的抗生物污染性能。此外，凝血酶结合适体被选为所提出的适体传感器的受体，它对 TB 具有优异的结合亲和力和选择性。当与 TB 结合时，在修饰电极界面处发生的电子转移被抑制，这可以归因于空间位阻效应，导致电流响应降低。该适体传感器具有宽检测范围和低检测限 0.031pM（S/N = 3）的优异电化学性能，具有高选择性、长期稳定性和良好的重现性。最后，通过我们提出的适体传感器直接实现了全血样品中 TB 的灵敏检测，表明其在临床 TB 检测中具有很大的应用潜力。
