Saland L C, Wallace J A, Samora A, Gutierrez L
Department of Anatomy, University of New Mexico School of Medicine, Albuquerque 87131.
Neurosci Lett. 1988 Nov 22;94(1-2):39-45. doi: 10.1016/0304-3940(88)90267-4.
Adult male Sprague-Dawley rats were examined for possible co-localization of tyrosine hydroxylase (TH) and serotonin (5-HT) within innervation of the pituitary neurointermediate lobe. Use of sheep antiserum to TH and a secondary antibody coupled to fluorescein isothiocyanate (FITC), followed by rabbit anti-5-HT, then rhodamine-coupled second antibody, produced co-existent staining in some, but not all fibers of both the neural and intermediate lobes, using paraffin embedded tissues. Application of a combination of the two primary antibodies followed by sequential application of secondary antibody also resulted in co-localized antigens in selected fibers. Multiple 'classic' neurotransmitters within the same nerve terminals may modulate selected areas of endocrine tissue to control hormone release.
对成年雄性斯普拉格-道利大鼠的垂体神经中间叶神经支配区域内酪氨酸羟化酶（TH）和5-羟色胺（5-HT）的可能共定位情况进行了检查。使用抗TH羊抗血清和与异硫氰酸荧光素（FITC）偶联的二抗，随后使用兔抗5-HT，再用罗丹明偶联的二抗，对石蜡包埋组织进行处理后，在神经叶和中间叶的部分（但不是全部）纤维中产生了共存染色。同时应用两种一抗，随后依次应用二抗，也在选定的纤维中产生了抗原共定位。同一神经末梢内的多种“经典”神经递质可能调节内分泌组织的特定区域以控制激素释放。
