Department of Medical Microbiology, Postgraduate Institute of Medical Education & Research, Chandigarh-160012, India.
Future Microbiol. 2017 Dec;12:1457-1466. doi: 10.2217/fmb-2017-0105. Epub 2017 Oct 27.
To standardize the matrix-assisted laser desorption ionization-time of flight mass spectrometry protocols and expansion of existing Bruker Biotyper database for mold identification.
MATERIALS & METHODS: Four different sample preparation methods (protocol A, B, C and D) were evaluated.
On analyzing each protein extraction method, reliable identification and best log scores were achieved through protocol D. The same protocol was used to identify 153 clinical isolates. Of these 153, 123 (80.3%) were accurately identified by using existing database and remaining 30 (19.7%) were not identified due to unavailability in database. On inclusion of missing main spectrum profile in existing database, all 153 isolates were identified.
Matrix-assisted laser desorption ionization-time of flight mass spectrometry can be used for routine identification of clinically important molds.
规范基质辅助激光解吸电离飞行时间质谱法协议，并扩展现有的布鲁克生物梅里翁数据库，以进行霉菌鉴定。
评估了四种不同的样品制备方法（方案 A、B、C 和 D）。
通过方案 D 分析每种蛋白质提取方法，可实现可靠的鉴定和最佳对数评分。相同的方案用于鉴定 153 株临床分离株。在这 153 株中，使用现有数据库准确鉴定了 123 株（80.3%），其余 30 株（19.7%）由于数据库中不存在而无法鉴定。在将缺失的主要光谱谱图纳入现有数据库后，所有 153 株分离株均被鉴定。
基质辅助激光解吸电离飞行时间质谱法可用于常规鉴定临床上重要的霉菌。
