Bao Riqiang, Xu Ping, Wang Yishu, Wang Jing, Xiao Li, Li Gang, Zhang Chunping
a Joint Programme of Nanchang University and Queen Mary University of London , Nanchang , Jiangxi , People's Republic of China.
b Second Clinical College , Nanchang University , Nanchang , Jiangxi , People's Republic of China.
Gynecol Endocrinol. 2018 Apr;34(4):320-326. doi: 10.1080/09513590.2017.1393661. Epub 2017 Oct 26.
Premature ovarian insufficiency (POI) is an important cause of infertility and also cause menopausal symptoms, which greatly reduced the quality of life for women. Hormone replacement therapy (HRT), as an important strategy, improved the quality of life for patients, however, the role of HRT in promoting fertility remains controversial. Therefore, seeking an optimal regime for POI becomes more urgent. In this study, we established POI model induced by CTX and BUS and utilized bone marrow derived mesenchymal stem cells (BM-MSCs) transplantation to treat the POI. We found that the decrease of estrogen and the increase of FSH induced by administration of CTX and BUS were rescued by BM-MSC transplantation. H&E staining and TUNEL assay showed that there were more healthy ovarian follicles and less apoptosis of ovarian cells after treatment with BM-MSCs. Further studies showed that there was an obvious decrease of Bax, p53, and p21 after transplantation, however, CyclinD2 was increased. In conclusion, our results demonstrated that BM-MSCs could restore injured ovarian function. Inhibiting apoptosis and promoting residual ovarian cell proliferation may contribute to the process.
卵巢早衰（POI）是不孕症的重要原因，还会引发更年期症状，这极大地降低了女性的生活质量。激素替代疗法（HRT）作为一项重要策略，改善了患者的生活质量，然而，HRT在促进生育方面的作用仍存在争议。因此，寻找针对POI的最佳治疗方案变得更加紧迫。在本研究中，我们建立了由环磷酰胺（CTX）和白消安（BUS）诱导的POI模型，并利用骨髓间充质干细胞（BM-MSCs）移植来治疗POI。我们发现，BM-MSC移植挽救了CTX和BUS给药所诱导的雌激素降低和促卵泡生成素（FSH）升高。苏木精-伊红（H&E）染色和TUNEL检测表明，BM-MSCs治疗后有更多健康的卵巢卵泡，卵巢细胞凋亡减少。进一步研究表明，移植后Bax、p53和p21明显减少，然而，细胞周期蛋白D2（CyclinD2）增加。总之，我们的结果表明BM-MSCs可以恢复受损的卵巢功能。抑制细胞凋亡和促进残留卵巢细胞增殖可能有助于这一过程。
