Department of Pharmaceutics and Tissue Engineering, School of Pharmacy, Jiangsu University, Zhenjiang, Xuefu Rd, 212013, People's Republic of China.
Sichuan Huahao Biotechnology Co. Ltd., Chengdu, 610041, People's Republic of China.
Stem Cell Res Ther. 2018 Apr 3;9(1):81. doi: 10.1186/s13287-018-0819-z.
Previous studies have reported that transplantation of mesenchymal stem cells (MSCs) from many human tissues could ameliorate ovarian dysfunction. However, no study has revealed the therapeutic efficiency of MSCs derived from the chorionic plate (CP-MSCs) for premature ovarian failure (POF).
We investigated the restorative effects of CP-MSCs on cyclophosphamide (CTX)-induced POF. The POF mouse models were established via intraperitoneal injection of 50 mg/kg CTX into female mice for 15 consecutive days. After that, CP-MSCs were intravenously transplanted into the mice once a week for 4 weeks. The serum estradiol (E2) and follicle-stimulating hormone (FSH) levels in the mouse models were detected using enzyme-linked immunosorbent assay (ELISA) before and after treatment. Ovarian function was evaluated through counting the follicles, estrous cycles, and oocytes.
CP-MSC transplantation restored the serum hormone level and ovarian function of the mice in the mouse model of POF induced by CTX. The levels of serum E2 and FSH in the POF model group was 232.33 ± 17.16 pg/mL and 4.48 ± 0.29 mIU/mL, respectively, after 6 weeks of treatment, which were similar to the values in the wild-type (WT) group. The superovulation demonstrated that ovarian function was significantly improved compared with nontreated POF model mice. The CP-MSC transplantation could restore CTX-induced ovarian dysfunction.
Our results offer a potential application for human CP-MSCs in POF treatment.
先前的研究报告称，源自多种人体组织的间充质干细胞（MSCs）移植可改善卵巢功能障碍。然而，尚无研究揭示源自胎盘绒毛板（CP-MSCs）的 MSCs 治疗卵巢早衰（POF）的疗效。
我们研究了 CP-MSCs 对环磷酰胺（CTX）诱导的 POF 的修复作用。通过连续 15 天向雌性小鼠腹腔内注射 50mg/kgCTX 建立 POF 小鼠模型。之后，每周对小鼠进行一次 CP-MSCs 静脉移植，共进行 4 周。采用酶联免疫吸附试验（ELISA）检测治疗前后小鼠模型血清雌二醇（E2）和卵泡刺激素（FSH）水平。通过计数卵泡、发情周期和卵母细胞来评估卵巢功能。
CP-MSC 移植可恢复 CTX 诱导的 POF 小鼠模型的血清激素水平和卵巢功能。POF 模型组治疗 6 周后血清 E2 和 FSH 水平分别为 232.33±17.16pg/ml 和 4.48±0.29mIU/ml，与野生型（WT）组相似。超排卵表明与未经治疗的 POF 模型小鼠相比，卵巢功能得到了显著改善。CP-MSC 移植可恢复 CTX 诱导的卵巢功能障碍。
我们的研究结果为 CP-MSCs 在 POF 治疗中的应用提供了一种潜在的方法。
