Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China; Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), Ministry of Agriculture, 100083, China; Beijing Laboratory for Food Quality and Safety, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
Regul Toxicol Pharmacol. 2017 Dec;91:226-234. doi: 10.1016/j.yrtph.2017.10.018. Epub 2017 Oct 23.
Canola is one of the most important plant oilseed crops. To avoid the threat of herbicides, the RF3 line with bar gene and barstar gene was developed, which can act as glufosinate resistance resources and restore fertility in hybrid lines. To assess the food safety of transgenic canola RF3, 2.5%, 5% and 10% GM canola RF3 and its non-GM isogenic line Drakkar were formulated into diet to feed Spragure-Dawley (SD) rats for 90 days. The effects on the general growth and toxicological parameters, as well as gut microbiota of rats, were evaluated. Several significant differences on body weight, feed consumption, relative organ weight, hematology and serum biochemistry were observed among rats in the 90-day feeding test. However, these statistical differences were randomly observed among different groups and were not dose-related, which were not considered to be biologically significant. Furthermore, the results of bacterial 16S rRNA sequencing of fecal samples showed that the diets containing GM canola did not disturb the balance of gut microbiota. In conclusion, the canola RF3 is considered as safe and wholesome as the non-GM canola based on this 90-day feeding test and gut microbiota analysis.
油菜籽是最重要的植物油料作物之一。为了避免除草剂的威胁，开发了携带 bar 基因和 barstar 基因的 RF3 系，它可以作为草甘膦抗性资源，并恢复杂种系的育性。为了评估转基因油菜 RF3 的食品安全，将 2.5%、5%和 10%的 GM 油菜 RF3 及其非 GM 同系物 Drakkar 配制成饲料，用于喂养 Spragure-Dawley（SD）大鼠 90 天。评估了对大鼠一般生长和毒理学参数以及肠道微生物群的影响。在 90 天喂养试验中，各组大鼠的体重、饲料消耗量、相对器官重量、血液学和血清生化指标均有显著差异。然而，这些统计学差异是随机观察到的不同组之间的，与剂量无关，不被认为具有生物学意义。此外，粪便样本的细菌 16S rRNA 测序结果表明，含有 GM 油菜的饮食并未破坏肠道微生物群的平衡。总之，基于这项 90 天喂养试验和肠道微生物群分析，油菜 RF3 被认为与非 GM 油菜一样安全和健康。
