Pharmaceutical Technology Department, National Research Centre, Dokki, Cairo 12622, Egypt.
Pharmaceutical Technology Department, National Research Centre, Dokki, Cairo 12622, Egypt.
Int J Pharm. 2017 Dec 20;534(1-2):325-338. doi: 10.1016/j.ijpharm.2017.10.044. Epub 2017 Oct 24.
The aim of the present study is to formulate the hydrophobic drug rutin in a solubilized form, intended for wound healing, via its loading into a novel Pickering emulsion stabilized by self aggregated chitosan particles (SACP). Rutin-loaded Pickering emulsion formulae were prepared using a high speed homogenizer. They were characterized by drop test, optical microscopy, droplet size and zeta potential determination. The results revealed that SACP have a nano size and a contact angle of 42.47±1.19° that tend to stabilize O/W emulsion. The droplet size of all investigated formulations ranged between 5.8±1.1 and 18.7±3.4μm. The long term stability study revealed that formulae containing 20% and 30% oily phase were stable against coalescence, the droplet size was slightly increased with zeta potential ranged from -48.1±4.7 to -78.4±4.1mV, during the storage period up to 5 months, indicating good stability. The release of rutin was almost 100% within 24h. Treatment of the wounded skin tissue of the Albino rats with the selected formula, for ten days, revealed almost complete healing. Biochemical analysis for oxidative stress markers, hyaluronic acid and collagen type I in addition to histopathological study were performed. The results suggested that the sustained release of rutin in a solubilized form as well as the synergistic effect of other components of the prepared Pickering emulsion could have a potential wound healing effect.
本研究的目的是通过将疏水性药物芦丁负载到由自聚集壳聚糖颗粒（SACP）稳定的新型 Pickering 乳液中，将其以溶解形式用于伤口愈合。使用高速匀浆机制备载有芦丁的 Pickering 乳液配方。通过滴落试验、光学显微镜、粒径和 Zeta 电位测定对其进行了表征。结果表明，SACP 的纳米尺寸和接触角为 42.47±1.19°，有利于稳定 O/W 乳液。所有研究配方的粒径均在 5.8±1.1 和 18.7±3.4μm 之间。长期稳定性研究表明，含有 20%和 30%油相的配方在聚集方面稳定，粒径略有增加，Zeta 电位范围从-48.1±4.7 到-78.4±4.1mV，在 5 个月的储存期内，表明稳定性良好。芦丁的释放在 24 小时内几乎达到 100%。用选定的配方治疗白化大鼠的受伤皮肤组织 10 天，发现几乎完全愈合。进行了氧化应激标志物、透明质酸和 I 型胶原蛋白的生化分析以及组织病理学研究。结果表明，芦丁以溶解形式的持续释放以及所制备的 Pickering 乳液的其他成分的协同作用可能具有潜在的伤口愈合作用。
