Blatt C, Aberdam D, Schwartz R, Sachs L
Department of Genetics, Weizmann Institute of Science, Rehovot, Israel.
EMBO J. 1988 Dec 20;7(13):4283-90. doi: 10.1002/j.1460-2075.1988.tb03326.x.
A homeobox gene rearrangement has been detected in WEHI-3B mouse myeloid leukaemic cells. The rearranged gene was identified as Hox-2.4 which is a member of the Hox-2 gene cluster on mouse chromosome 11. Both the normal and the rearranged genes were cloned and analysed, and the rearranged genomic Hox-2.4 gene was sequenced. The results indicate that the rearrangement is due to insertion of an intracisternal A particle 5' upstream to Hox-2.4 and that this resulted in constitutive expression of the homeobox gene. It is suggested that constitutive expression of the homeobox gene may interrupt the normal development program in these leukaemic cells.
在WEHI-3B小鼠髓性白血病细胞中检测到一种同源框基因重排。重排后的基因被鉴定为Hox-2.4，它是小鼠11号染色体上Hox-2基因簇的成员。对正常基因和重排基因都进行了克隆和分析，并对重排后的基因组Hox-2.4基因进行了测序。结果表明，这种重排是由于一个脑池内A颗粒插入到Hox-2.4基因5'上游所致，这导致了同源框基因的组成性表达。有人提出，同源框基因的组成性表达可能会中断这些白血病细胞的正常发育程序。
