Ok Bozkaya Ikbal, Yarali Nese, Kizilgün Murat, Ozkan Secil, Tunc Bahattin
Department of Pediatric Hematology, Ankara Children's Hematology Oncology Hospital, Kurtdereli Sokak, Irfan Bastug Cad. No: 10, 06110 Diskapi, Ankara, Turkey.
Department of Biochemistry, Ankara Children's Hematology Oncology Hospital, Ankara, Turkey.
Indian J Hematol Blood Transfus. 2017 Dec;33(4):537-540. doi: 10.1007/s12288-017-0789-9. Epub 2017 Feb 9.
The purpose of this study was to evaluate the relationship between the plasma holoTC and serum vitamin B in children and to identify a cutoff cobalamin values according to holoTC. One hundred and fifty-five children were enrolled into the study. All children were evaluated for hemoglobin, vitamin B, folate, ferritin and holoTC levels. Children were grouped as with low vitamin B level (≤200 pg/mL, group I) and normal vitamin B (>200 pg/mL, group II). Serum vitamin B, and holoTC levels were performed in each patient in the study. In 101 patients with low vitamin B (group I) the mean holoTC was 21.74 ± 1.14 pmol/L. In 54 children with normal vitamin B (group II) mean holoTC was 44.0 ± 2.7 pmol/L ( < 0.01). A ROC curve analysis was performed to delineate the optimum cut-off point for vitamin B level and it was found to be 165 pg/mL with a sensitivity of 70% and specificity of 74%; the area under curve was 0.783 ( < 0.01). Our study displayed a positive correlation between vitamin B and holoTC, and defined an optimum cutoff value for vitamin B as 165 pg/mL. Further studies using the markers both MMA, tHcy and holoTC to confirm the findings are needed.
本研究的目的是评估儿童血浆全转钴胺素（holoTC）与血清维生素B之间的关系，并根据holoTC确定钴胺素的临界值。155名儿童纳入本研究。所有儿童均接受血红蛋白、维生素B、叶酸、铁蛋白和holoTC水平评估。儿童被分为维生素B水平低（≤200 pg/mL，I组）和维生素B正常（>200 pg/mL，II组）。研究中对每位患者进行血清维生素B和holoTC水平检测。101名维生素B水平低的患者（I组），全转钴胺素平均水平为21.74±1.14 pmol/L。54名维生素B正常的儿童（II组），全转钴胺素平均水平为44.0±2.7 pmol/L（P<0.01）。进行ROC曲线分析以确定维生素B水平的最佳临界点，发现为165 pg/mL，敏感性为70%，特异性为74%；曲线下面积为0.783（P<0.01）。我们的研究显示维生素B与全转钴胺素之间呈正相关，并确定维生素B的最佳临界值为165 pg/mL。需要进一步研究使用甲基丙二酸（MMA）、总同型半胱氨酸（tHcy）和全转钴胺素这些标志物来证实这些发现。
