Zhu Wei, Chi Nan, Zou Peng, Chen Hongguang, Tang Guotai, Zhao Wei
Department of Neurosurgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China.
Exp Ther Med. 2017 Nov;14(5):4411-4416. doi: 10.3892/etm.2017.5054. Epub 2017 Aug 28.
The present study aimed to investigate the protective effects of docosahexaenoic acid (DHA) on traumatic brain injury (TBI) in rats. A model of TBI was induced by lateral fluid percussion injury in adult rats and rats were randomly divided into the TBI-model group, TBI-low DHA group and TBI-high DHA group, while other healthy rats were assigned to the sham-operated group. Motor recovery was tested with beam-walking trials at 2, 7 and 15 days post-TBI. Cognitive recovery was tested with Morris water maze trials at 15 days post-TBI. The expression levels of caspase-3, B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) were measured by western blotting. DHA protected against motor deficits induced by TBI in beam walking tests. All TBI-model groups had longer escape latency and swimming distances than the sham groups. Compared with the TBI-low DHA group, the TBI-high DHA group demonstrated shorter escape latency and swimming distances. DHA inhibited the expression of caspase-3 and the inhibition effect was more obvious at a high dosage. Furthermore, DHA dose-dependently rescued neurons by upregulating the Bcl-2:Bax ratio. DHA supplementation was a viable strategy to mitigate injury from TBI.
本研究旨在探讨二十二碳六烯酸（DHA）对大鼠创伤性脑损伤（TBI）的保护作用。采用成年大鼠侧方液压冲击伤诱导TBI模型，将大鼠随机分为TBI模型组、TBI低剂量DHA组和TBI高剂量DHA组，同时将其他健康大鼠分配到假手术组。在TBI后2天、7天和15天通过横梁行走试验测试运动恢复情况。在TBI后15天通过莫里斯水迷宫试验测试认知恢复情况。通过蛋白质免疫印迹法检测半胱天冬酶-3、B细胞淋巴瘤2（Bcl-2）和Bcl-2相关X蛋白（Bax）的表达水平。在横梁行走试验中，DHA可预防TBI诱导的运动功能障碍。所有TBI模型组的逃避潜伏期和游泳距离均比假手术组长。与TBI低剂量DHA组相比，TBI高剂量DHA组的逃避潜伏期和游泳距离更短。DHA抑制半胱天冬酶-3的表达，且高剂量时抑制作用更明显。此外，DHA通过上调Bcl-2:Bax比值剂量依赖性地挽救神经元。补充DHA是减轻TBI损伤的一种可行策略。
