Laboratory of Molecular Pathology, Department of Pathology and Diagnostics, University Hospital of Verona, Verona, Italy.
Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy.
J Neurooncol. 2018 Jan;136(1):51-62. doi: 10.1007/s11060-017-2639-x. Epub 2017 Oct 26.
Malignant gliomas, the most frequent primary brain tumors, are characterized by a dismal prognosis. Reliable biomarkers complementary to neuroradiology in the differential diagnosis of gliomas and monitoring for post-surgical progression are unmet needs. Altered expression of several microRNAs in tumour tissues from patients with gliomas compared to normal brain tissue have been described, thus supporting the rationale of using microRNA-based biomarkers. Although different circulating microRNAs were proposed in association with gliomas, they have not been introduced into clinical practice so far. Blood samples were collected from patients with high and low grade gliomas, both before and after surgical resection, and the expression of miR-21, miR-222 and miR-124-3p was measured in exosomes isolated from serum. The expression levels of miR-21, miR-222 and miR-124-3p in serum exosomes of patients with high grade gliomas were significantly higher than those of low grade gliomas and healthy controls and were sharply decreased in samples obtained after surgery. The analysis of miR-21, miR-222 and miR-124-3p in serum exosomes of patients affected by gliomas can provide a minimally invasive and innovative tool to help the differential diagnosis of gliomas at their onset in the brain and predict glioma grading and non glial metastases before surgery.
恶性胶质瘤是最常见的原发性脑肿瘤，其预后较差。在胶质瘤的鉴别诊断和术后进展监测方面，神经影像学的补充可靠生物标志物是未满足的需求。与正常脑组织相比，来自胶质瘤患者的肿瘤组织中几种 microRNA 的表达发生改变，这支持了使用基于 microRNA 的生物标志物的原理。尽管已经提出了与胶质瘤相关的不同循环 microRNA，但迄今为止尚未将其引入临床实践。从高级别和低级别胶质瘤患者采集术前和术后的血液样本，并测量从血清中分离出的外泌体中的 miR-21、miR-222 和 miR-124-3p 的表达。高级别胶质瘤患者血清外泌体中 miR-21、miR-222 和 miR-124-3p 的表达水平明显高于低级别胶质瘤和健康对照组，并且在手术后获得的样本中急剧降低。对受胶质瘤影响的患者血清外泌体中 miR-21、miR-222 和 miR-124-3p 的分析可以提供一种微创且创新的工具，有助于在脑内起始时对胶质瘤进行鉴别诊断，并在手术前预测胶质瘤分级和非神经胶质转移。
