Wang Zhi-Dan, Yan Ning, Wang Zheng-Hong, Zhang Xiao-Huan, Zhang Jing-Ze, Xue Hui-Min, Wang Li-Xia, Zhan Qi, Xu Ying-Ping, Guo De-Ping
Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Plant Mol Biol. 2017 Dec;95(6):533-547. doi: 10.1007/s11103-017-0658-9. Epub 2017 Oct 26.
We report a transcriptome assembly and expression profiles from RNA-Seq data and identify genes responsible for culm gall formation in Zizania latifolia induced by Ustilago esculenta. The smut fungus Ustilago esculenta can induce culm gall in Zizania latifolia, which is used as a vegetable in Asian countries. However, the underlying molecular mechanism of culm gall formation is still unclear. To characterize the processes underlying this host-fungus association, we performed transcriptomic and expression profiling analyses of culms from Z. latifolia infected by the fungus U. esculenta. Transcriptomic analysis detected U. esculenta induced differential expression of 19,033 and 17,669 genes in Jiaobai (JB) and Huijiao (HJ) type of gall, respectively. Additionally, to detect the potential gall inducing genes, expression profiles of infected culms collected at -7, 1 and 10 DAS of culm gall development were analyzed. Compared to control, we detected 8089 genes (4389 up-regulated, 3700 down-regulated) and 5251 genes (3121 up-regulated, 2130 down-regulated) were differentially expressed in JB and HJ, respectively. And we identified 376 host and 187 fungal candidate genes that showed stage-specific expression pattern, which are possibly responsible for gall formation at the initial and later phases, respectively. Our results indicated that cytokinins play more prominent roles in regulating gall formation than do auxins. Together, our work provides general implications for the understanding of gene regulatory networks for culm gall development in Z. latifolia, and potential targets for genetic manipulation to improve the future yield of this crop.
我们报告了基于RNA测序数据的转录组组装和表达谱，并鉴定了由茭白黑粉菌诱导的菰茎形成虫瘿的相关基因。茭白黑粉菌可在菰中诱导茎部形成虫瘿，在亚洲国家菰被用作蔬菜。然而，茎部形成虫瘿的潜在分子机制仍不清楚。为了阐明这种宿主 - 真菌相互作用的潜在机制，我们对受茭白黑粉菌感染的菰茎进行了转录组和表达谱分析。转录组分析分别在茭白（JB）型和灰茭（HJ）型虫瘿中检测到茭白黑粉菌诱导19,033个和17,669个基因差异表达。此外，为了检测潜在的虫瘿诱导基因，我们分析了在茎部虫瘿发育的第 -7、1和10天收集的受感染茎的表达谱。与对照相比，我们分别在JB和HJ中检测到8089个基因（4389个上调，3700个下调）和5251个基因（3121个上调，2130个下调）差异表达。我们鉴定出376个宿主和187个真菌候选基因，它们分别呈现阶段特异性表达模式，可能分别在虫瘿形成的初始阶段和后期阶段发挥作用。我们的结果表明，细胞分裂素在调节虫瘿形成中比生长素发挥更显著的作用。总之，我们的工作为理解菰茎部虫瘿发育的基因调控网络提供了一般性启示，并为通过基因操作提高该作物未来产量提供了潜在靶点。
