Navarro Roge M, Pino Ander, Martinez-Andres Asunción, Molina Consuelo, Martinez Ana María, Martinez Nahikari, Orive Gorka, Anitua Eduardo
Centro Dermatológico-Estético de Alicante, Alicante, Spain.
BTI Biotechnology Institute, Vitoria (Álava), Spain.
J Cosmet Dermatol. 2018 Oct;17(5):862-873. doi: 10.1111/jocd.12446. Epub 2017 Oct 26.
Hair transplant surgery using follicular unit extraction technique (FUE) is a common surgical procedure for the treatment of severe hair loss. Blood-derived autologous growth factors have also proved to promote hair regeneration in patients with different types of alopecia.
The aim of this study was to evaluate the safety and clinical efficacy of plasma rich in growth factors (PRGF) technology as an adjuvant therapy for FUE surgery in hair loss affected patients.
The biologic potential of PRGF was firstly in vitro evaluated over follicular germinal matrix and dermal papilla cells. Afterward, fifteen patients were subjected to routine FUE procedure while 15 patients underwent FUE+PRGF therapy. PRGF group included intradermal injections of growth factors and follicular transfer unit (FTU) preservation in an autologous fibrin clot. Postsurgical patient satisfaction and clinical improvement were evaluated, and PRGF or saline-preserved hair grafts were histomorphometrically analyzed.
Follicular cell proliferation and migration was induced after autologous growth factors treatment. PRGF-preserved FTUs presented higher bioactivity signals and improved integrity of perifollicular structures and extracellular matrix proteins such as collagen and elastic fibers. PRGF not only reduced the postsurgical crust healing and hair fixation period, but also decreased the inflammatory pain and itching sensation.
This preliminary data demonstrate that PRGF is able to minimize the postsurgical follicle loss and potentiate the performance of grafted hairs. The fibrin clot not only acts as a protective barrier against environmental factors, but also provides a biologically active scaffold that induces resident cell proliferation and maintains an optimal integrity of the grafted hair.
采用毛囊单位提取技术（FUE）的毛发移植手术是治疗严重脱发的常见外科手术。血液来源的自体生长因子也已被证明可促进不同类型脱发患者的头发生长。
本研究的目的是评估富含生长因子的血浆（PRGF）技术作为脱发患者FUE手术辅助治疗的安全性和临床疗效。
首先在体外评估PRGF对毛囊生发基质和真皮乳头细胞的生物学潜力。之后，15例患者接受常规FUE手术，而15例患者接受FUE+PRGF治疗。PRGF组包括皮内注射生长因子和将毛囊移植单位（FTU）保存在自体纤维蛋白凝块中。评估术后患者满意度和临床改善情况，并对PRGF或生理盐水保存的毛发移植物进行组织形态计量学分析。
自体生长因子治疗后诱导了毛囊细胞增殖和迁移。PRGF保存的FTU呈现出更高的生物活性信号，并改善了毛囊周围结构和细胞外基质蛋白（如胶原蛋白和弹性纤维）的完整性。PRGF不仅缩短了术后结痂愈合和毛发固定期，还减轻了炎症性疼痛和瘙痒感。
这些初步数据表明，PRGF能够将术后毛囊损失降至最低，并增强移植毛发的性能。纤维蛋白凝块不仅作为抵御环境因素的保护屏障，还提供了一个生物活性支架，可诱导驻留细胞增殖并维持移植毛发的最佳完整性。
