Department of Chemistry, Faculty of Biotechnology and Food Science, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland.
Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, Jagiellonska 28, 40-032 Katowice, Poland.
Molecules. 2017 Oct 27;22(11):1830. doi: 10.3390/molecules22111830.
A group of flavones, isoflavones, flavanones, and chalcones was subjected to small-scale biotransformation studies with the Gram-negative KB2 strain in order to evaluate the capability of this strain to transform flavonoid compounds and to investigate the relationship between compound structure and transformation type. The tested strain transformed flavanones and chalcones. The main type of transformation of compounds with a flavanone moiety was central heterocyclic C ring cleavage, leading to chalcone and dihydrochalcone structures, whereas chalcones underwent reduction to dihydrochalcones and cyclisation to a benzo-γ-pyrone moiety. Substrates with a C-2-C-3 double bond (flavones and isoflavones) were not transformed by KB2.
一组黄酮类、异黄酮类、黄烷酮类和查尔酮类化合物在革兰氏阴性 KB2 菌株的小规模生物转化研究中进行了测试，以评估该菌株转化黄酮类化合物的能力，并研究化合物结构与转化类型之间的关系。测试菌株转化了黄烷酮类和查尔酮类化合物。具有黄烷酮部分的化合物的主要转化类型是中环杂原子 C 环裂解，导致查尔酮和二氢查尔酮结构，而查尔酮则被还原为二氢查尔酮并环化为苯并-γ-吡喃酮部分。KB2 不能转化具有 C-2-C-3 双键的底物（黄酮类和异黄酮类）。
