Piotrowski Carolin, Lede Vera, Butthof Anne, Kaiser Nicole, Hirrlinger Petra G, Tschöp Matthias H, Schöneberg Torsten, Bechmann Ingo
Institute of Anatomy, Faculty of Medicine, University of Leipzig, Leipzig, Germany.
Rudolf Schönheimer Institute of Biochemistry, Faculty of Medicine, University of Leipzig, Leipzig, Germany.
PLoS One. 2017 Oct 27;12(10):e0187192. doi: 10.1371/journal.pone.0187192. eCollection 2017.
Nasal mucosa and olfactory bulb are separated by the cribriform plate which is perforated by olfactory nerves. We have previously demonstrated that the cribriform plate is permissive for T cells and monocytes and that viruses can enter the bulb upon intranasal injection by axonal transportation. Therefore, we hypothesized that nasal mucosa and olfactory bulb are equipped to deal with constant infectious threats. To detect genes involved in this process, we compared gene expression in nasal mucosa and bulb of mice kept under specific pathogen free (SPF) conditions to gene expression of mice kept on non-SPF conditions using RNA deep sequencing. We found massive alterations in the expression of immune-related genes of the nasal mucosa, while the bulb did not respond immunologically. The absence of induction of immune-related genes in the olfactory bulb suggests effective defence mechanisms hindering entrance of environmental pathogens beyond the outer arachnoid layer. The genes detected in this study may include candidates conferring susceptibility to meningitis.
鼻黏膜和嗅球被筛板分隔开，嗅神经穿过筛板。我们之前已经证明，筛板允许T细胞和单核细胞通过，并且病毒经鼻内注射后可通过轴突运输进入嗅球。因此，我们推测鼻黏膜和嗅球具备应对持续感染威胁的能力。为了检测参与这一过程的基因，我们使用RNA深度测序技术，将饲养在特定病原体-free（SPF）条件下的小鼠鼻黏膜和嗅球中的基因表达与饲养在非SPF条件下的小鼠的基因表达进行了比较。我们发现鼻黏膜中免疫相关基因的表达发生了大量改变，而嗅球在免疫方面没有反应。嗅球中未诱导免疫相关基因表明存在有效的防御机制，可阻碍环境病原体进入蛛网膜外层之外。本研究中检测到的基因可能包括导致脑膜炎易感性的候选基因。
