Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, Pakistan.
School of Interdisciplinary Engineering & Sciences, National University of Sciences and Technology, Islamabad, Pakistan.
Sci Rep. 2023 May 9;13(1):7551. doi: 10.1038/s41598-023-32023-7.
Plants require copper for normal growth and development and have evolved an efficient system for copper management based on transport proteins such as P-ATPases, also known as heavy metal ATPases (HMAs). Here, we report HMAs in eleven different Poaceae species, including wheat. Furthermore, the possible role of wheat HMAs in copper stress was investigated. BlastP searches identified 27 HMAs in wheat, and phylogenetic analysis based on the Maximum Likelihood method demonstrated a separation into four distinct clades. Conserved motif analysis, domain identification, gene structure, and transmembrane helices number were also identified for wheat HMAs using computational tools. Wheat seedlings grown hydroponically were subjected to elevated copper and demonstrated toxicity symptoms with effects on fresh weight and changes in expression of selected HMAs TaHMA7, TaHMA8, and TaHMA9 were upregulated in response to elevated copper, suggesting a role in wheat copper homeostasis. Further investigations on these heavy metal pumps can provide insight into strategies for enhancing crop heavy metal tolerance in the face of heavy metal pollution.
植物的正常生长和发育需要铜，并且已经进化出了一种基于转运蛋白（如 P-ATPase，也称为重金属 ATP 酶（HMAs））的有效的铜管理系统。在这里，我们报告了 11 种不同禾本科物种中的 HMAs，包括小麦。此外，还研究了小麦 HMAs 在铜胁迫下的可能作用。BlastP 搜索鉴定了小麦中的 27 个 HMA，基于最大似然法的系统发育分析表明它们分为四个不同的分支。还使用计算工具对小麦 HMAs 进行了保守基序分析、结构域鉴定、基因结构和跨膜螺旋数的鉴定。水培生长的小麦幼苗受到高浓度铜的影响，表现出毒性症状，对鲜重有影响，并且选择的 HMAs TaHMA7、TaHMA8 和 TaHMA9 的表达发生变化，表明它们在小麦铜稳态中起作用。对这些重金属泵的进一步研究可以为在重金属污染下增强作物对重金属的耐受性提供策略。
