Nakayama H, Iohara K, Hayashi Y, Okuwa Y, Kurita K, Nakashima M
Department of Stem Cell Biology and Regenerative Medicine, National Center for Geriatrics and Gerontology, Research Institute, Obu, Japan.
Department of Oral Maxillofacial Surgery, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Oral Dis. 2017 Jul;23(5):620-628. doi: 10.1111/odi.12619. Epub 2017 Apr 5.
We have previously demonstrated that dental pulp stem cells (DPSCs) isolated from mature teeth by granulocyte colony-stimulating factor (G-CSF)-induced mobilization method can enhance angiogenesis/vasculogenesis and improve pulp regeneration when compared with colony-derived DPSCs. However, the efficacy of this method in immature teeth with root-formative stage has never been investigated. Therefore, the aim of this study was to examine the stemness, biological characteristics, and regeneration potential in mobilized DPSCs compared with colony-derived DPSCs from immature teeth.
Mobilized DPSCs isolated from immature teeth were compared to colony-derived DPSCs using methods including flow cytometry, migration assays, mRNA expression of angiogenic/neurotrophic factor, and induced differentiation assays. They were also compared in trophic effects of the secretome. Regeneration potential was further compared in an ectopic tooth transplantation model.
Mobilized DPSCs had higher migration ability and expressed more angiogenic/neurotrophic factors than DPSCs. The mobilized DPSC secretome produced a higher stimulatory effect on migration, immunomodulation, anti-apoptosis, endothelial differentiation, and neurite extension. In addition, vascularization and pulp regeneration potential were higher in mobilized DPSCs than in DPSCs.
G-CSF-induced mobilization method enhances regeneration potential of colony-derived DPSCs from immature teeth.
我们之前已经证明，通过粒细胞集落刺激因子（G-CSF）诱导动员法从成熟牙齿中分离出的牙髓干细胞（DPSCs），与集落衍生的DPSCs相比，能够增强血管生成/血管发生并改善牙髓再生。然而，该方法在处于牙根形成期的未成熟牙齿中的效果尚未得到研究。因此，本研究的目的是比较动员的DPSCs与从未成熟牙齿中分离的集落衍生DPSCs的干性、生物学特性和再生潜力。
使用包括流式细胞术、迁移试验、血管生成/神经营养因子的mRNA表达以及诱导分化试验等方法，将从未成熟牙齿中分离出的动员DPSCs与集落衍生的DPSCs进行比较。还对它们的分泌组的营养作用进行了比较。在异位牙移植模型中进一步比较了再生潜力。
动员的DPSCs比DPSCs具有更高的迁移能力，并且表达更多的血管生成/神经营养因子。动员的DPSC分泌组对迁移、免疫调节、抗凋亡、内皮分化和神经突延伸产生更高的刺激作用。此外，动员的DPSCs的血管化和牙髓再生潜力高于DPSCs。
G-CSF诱导动员法增强了从未成熟牙齿中分离的集落衍生DPSCs的再生潜力。
