O'Donnell Neil, Dmitriev Ruslan I
Metabolic Imaging Group, School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland.
Adv Exp Med Biol. 2017;1035:49-67. doi: 10.1007/978-3-319-67358-5_4.
In recent years, the advances in tissue engineering and regenerative medicine have resulted in introduction of novel 3D tissue models, materials and methods to the regular practice of cell biologists, material scientists and specialists from related areas. 3D tissue models allow mimicking in vivo cell and tissue organization. However, the efficient work in three dimensions has significant challenges, such as compatibility with conventional cell biology methods, live cell imaging and quantification readouts. Here, we briefly discuss the applicability of 3D tissue models to different live cell microscopy modalities and the available range of fluo- and phosphorescent probes and sensors allowing for multi-parametric imaging.
近年来，组织工程和再生医学的进展促使新型三维组织模型、材料和方法被引入细胞生物学家、材料科学家及相关领域专家的常规实践中。三维组织模型能够模拟体内细胞和组织的结构。然而，在三维空间中的高效研究面临重大挑战，例如与传统细胞生物学方法的兼容性、活细胞成像以及定量读数。在此，我们简要讨论三维组织模型在不同活细胞显微镜检查方式中的适用性，以及适用于多参数成像的荧光和磷光探针及传感器的可用范围。
