Le Marois Alix, Suhling Klaus
Department of Physics, King's College London, Strand, London, WC2R 2LS, UK.
Adv Exp Med Biol. 2017;1035:31-48. doi: 10.1007/978-3-319-67358-5_3.
In this chapter, the concept of fluorescence lifetime and its utility in quantitative live cell imaging will be introduced, along with methods to record and analyze FLIM data. Relevant applications in 3D tissue and live cell imaging, including multiplexed FLIM detection, will also be detailed.
在本章中，将介绍荧光寿命的概念及其在定量活细胞成像中的应用，以及记录和分析荧光寿命成像（FLIM）数据的方法。还将详细介绍其在三维组织和活细胞成像中的相关应用，包括多重FLIM检测。
