Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
Department of Maternal and Perinatal Medicine, Nagoya University Hospital, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
J Assist Reprod Genet. 2018 Feb;35(2):339-343. doi: 10.1007/s10815-017-1073-5. Epub 2017 Oct 27.
To visualize and analyze follicle development in ovarian tissue culture using physiological concentrations of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in order to establish an ovarian tissue culture system that enables efficient in vitro growth of follicles.
Ovarian tissues from 4-week-old female ICR mice were sliced and cultured. Images of ovarian tissues in culture were obtained at 24-h or 30-min intervals by using a microscope. The area of each follicle observed in the ovarian tissue slices was tracked and analyzed in association with oocyte maturation.
We were able to track the development of each follicle using this culture system. Follicle growth was associated with oocyte maturation. Meiotically matured oocytes (MII) were obtained from 33% of all follicles investigated. Approximately, a quarter of follicles (24%) did not grow and resulted in atresia.
Follicle dynamics were successfully visualized and analyzed in murine ovarian tissue culture. We were able to obtain mature oocytes from the fully grown follicles in vitro. This culture system would be helpful for efficient in vitro culturing of ovarian tissues.
使用生理浓度的卵泡刺激素（FSH）和黄体生成素（LH）在卵巢组织培养中可视化和分析卵泡发育，以建立一种能够有效体外生长卵泡的卵巢组织培养系统。
将 4 周龄雌性 ICR 小鼠的卵巢组织切片并培养。使用显微镜以 24 小时或 30 分钟的间隔获取卵巢组织培养物的图像。跟踪和分析卵巢组织切片中每个卵泡的面积与卵母细胞成熟有关。
我们能够使用这种培养系统跟踪每个卵泡的发育。卵泡生长与卵母细胞成熟有关。从所有研究的卵泡中获得了 33%的有丝分裂成熟卵母细胞（MII）。大约四分之一的卵泡（24%）没有生长，导致闭锁。
成功地在鼠类卵巢组织培养中可视化和分析了卵泡动力学。我们能够从体外完全生长的卵泡中获得成熟的卵母细胞。这种培养系统将有助于卵巢组织的高效体外培养。
