Patki Rucha, Lilani Sunil, Lanjewar Dhaneshwar
Grant Government Medical College and Sir J. J. Group of Hospitals, Mumbai, Maharashtra, India.
Shri Bhausaheb Hire Government Medical College and Hospital, Dhule, India.
Int J Microbiol. 2017;2017:9042125. doi: 10.1155/2017/9042125. Epub 2017 Aug 31.
The aim of this study was to establish a baseline titre for the population of Mumbai, Maharashtra, India.
Four hundred healthy blood donors, attending blood donation camps, were screened using a survey questionnaire. Widal tube agglutination test was performed on the diluted sera (with 0.9% normal saline) of blood donors, with final dilution ranging from 1 : 40 to 1 : 320.
Out of 400 individuals providing samples, 78 (19.5%) individuals showed antibody titres ≥ 1 : 40 for at least one antigen and 322 (80.5%) showed no agglutination. The baseline antibody titres against O antigen and H antigen of serotype Typhi were found to be 1 : 40 and 1 : 80, respectively. Similarly, the baseline antibody titres for the H antigen of serotypes Paratyphi A and Paratyphi B were found to be 1 : 40 and 1 : 80, respectively.
Thus, it was noted that the diagnostically significant cutoff of antibody titre from acute phase sample was ≥ 1 : 80 for Typhi O antigen and titre of ≥ 1 : 160 for both Typhi H antigen and Paratyphi BH antigen. Antibody titre of ≥ 1 : 80 can be considered significant for Paratyphi AH antigen.
本研究旨在确定印度马哈拉施特拉邦孟买人群的基线滴度。
使用调查问卷对400名前往献血营的健康献血者进行筛查。对献血者的稀释血清（用0.9%生理盐水）进行肥达氏试管凝集试验，最终稀释度范围为1∶40至1∶320。
在提供样本的400人中，78人（19.5%）至少对一种抗原的抗体滴度≥1∶40，322人（80.5%）未出现凝集。伤寒血清型O抗原和H抗原的基线抗体滴度分别为1∶40和1∶80。同样，甲型副伤寒和乙型副伤寒血清型H抗原的基线抗体滴度分别为1∶40和1∶80。
因此，注意到急性期样本中抗体滴度的诊断意义临界值对于伤寒O抗原为≥1∶80，对于伤寒H抗原和乙型副伤寒H抗原均为≥1∶160。甲型副伤寒H抗原的抗体滴度≥1∶80可被认为具有意义。
