El-Olemy G M, Atta A A, Mahmoud W H, Hamzah E G
Dev Biol Stand. 1984;56:565-72.
50 Blood samples were collected from in-patients with febrile splenomegaly suspected to be Malta fever, in Sharkia fever hospitals; Egypt. The samples were subjected to serological tests (tube agglutination, Widal & Coombs) for diagnosis of brucellosis. The results of tube agglutination (TAT) revealed that 5 (10%) were positive; the titre ranging from 1/80-1/320, 3 doubtful (1/40) and 42 negative (1/20, 1/10 and no agglutination). Results of the Coombs test on negative and doubtful of TAT showed that out of 45 patients; 3 (6.66%) were positive; the titre ranging from 1/80-1/160, one doubtful and 41 negative. Coombs test improved the titre of one positive case of TAT from 1/320-1/640. Results of serological tests (TAT & Coombs) for MALTA fever were 8 (16%); the titre ranging from 1/80-1/640, one doubtful (1/40) and 41 negative. Results of the Widal test to detect Enteric fever in the way of differential diagnosis were 7 (14%) positive out of 50 patients examined; the titre ranging from 1/80-1/160. Five of them agglutinated typhoid (O&M) suspensions. One of the sera agglutinated paratyphi A (H) suspension, the remaining one serum agglutinated paratyphi. B(H) suspension and none of the sera agglutinated paratyphi. C(H) suspension. All the positive Coombs and negative Widal cases were obtained against typhoid (O) suspension and none against any of the (H) suspensions; Coombs test was positive in two (4.65) of 43 patients negative to Widal; no improvement of titre in the positive Widal. Results of Widal & Coombs to detect Enterica were 9 (18%) positive and 41 negative.
在埃及谢克希亚热病医院，从疑似患马耳他热的发热性脾肿大住院患者中采集了50份血样。对这些样本进行血清学检测（试管凝集试验、肥达氏试验和库姆斯试验）以诊断布鲁氏菌病。试管凝集试验（TAT）结果显示，5份（10%）呈阳性；滴度范围为1/80 - 1/320，3份可疑（1/40），42份阴性（1/20、1/10且无凝集反应）。对TAT阴性和可疑样本进行的库姆斯试验结果显示，在45名患者中，3份（6.66%）呈阳性；滴度范围为1/80 - 1/160，1份可疑，41份阴性。库姆斯试验将1例TAT阳性病例的滴度从1/320提高到了1/640。马耳他热血清学检测（TAT和库姆斯试验）结果为8份（16%）呈阳性；滴度范围为1/80 - 1/640，1份可疑（1/40），41份阴性。在用于鉴别诊断检测肠热病的肥达氏试验结果中，在所检测的50名患者中有7份（14%）呈阳性；滴度范围为1/80 - 1/160。其中5份凝集了伤寒（O&M）悬液。1份血清凝集了副伤寒A（H）悬液，其余1份血清凝集了副伤寒B（H）悬液，没有血清凝集副伤寒C（H）悬液。所有库姆斯试验阳性且肥达氏试验阴性的病例均针对伤寒（O）悬液出现反应，针对任何（H）悬液均无反应；在43名肥达氏试验阴性的患者中，有2名（4.65%）库姆斯试验呈阳性；肥达氏试验阳性的病例滴度无提高。检测肠道菌的肥达氏试验和库姆斯试验结果为9份（18%）呈阳性，41份阴性。
