Hagan I, Hayles J, Nurse P
Department of Biochemistry, University of Oxford.
J Cell Sci. 1988 Dec;91 ( Pt 4):587-95. doi: 10.1242/jcs.91.4.587.
We have cloned and sequenced the cdc13+ gene from fission yeast. When a major part of the cdc13+ gene is deleted from the chromosome, cells arrest in interphase, but partial loss of gene activity leads to cells containing condensed chromosomes, aberrant septa and a microtubular cytoskeleton with characteristics of both G2 and M. Expression of this phenotype is influenced by the nutritional status of the cell. Our results suggest that the cdc13+ gene function is required for the control of the G2 to M transition. It appears to play a role in regulating the separate pathways of events involved in the physical process of mitosis, for example in the reorganization of the cytoskeleton on transition from G2 to mitosis. The cdc13+ gene function interacts closely with both the yeast and human homologues of cdc2+, suggesting that mammalian cells may contain a cdc13+ homologue. The gene encodes a putative polypeptide of 482 amino acids, and a central region of 176 amino acids of this polypeptide is 50% identical with sea urchin cyclin. Therefore, the cdc13+ protein is cyclin related and could act as a regulator or substrate of the p34cdc2 protein kinase, which initiates mitosis.
我们已经从裂殖酵母中克隆并测序了cdc13+基因。当该基因的大部分从染色体上缺失时，细胞停滞在间期，但基因活性的部分丧失会导致细胞含有浓缩染色体、异常隔膜以及具有G2期和M期特征的微管细胞骨架。这种表型的表达受细胞营养状态的影响。我们的结果表明，cdc13+基因功能是控制从G2期到M期转变所必需的。它似乎在调节有丝分裂物理过程中涉及的不同事件途径中发挥作用，例如在从G2期到有丝分裂转变时细胞骨架的重组。cdc13+基因功能与cdc2+的酵母和人类同源物密切相互作用，这表明哺乳动物细胞可能含有cdc13+同源物。该基因编码一个推定的482个氨基酸的多肽，该多肽的176个氨基酸的中央区域与海胆细胞周期蛋白有50%的同一性。因此，cdc13+蛋白与细胞周期蛋白相关，可能作为启动有丝分裂的p34cdc2蛋白激酶的调节因子或底物。
