Ultra-High-Throughput Analytical Strategy Based on UHPLC-DAD in Combination with Syringe Filtration for the Quantitation of Nine Synthetic Colorants in Beverages: Impacts of Syringe Membrane Types and Sample pH on Recovery.

An ultra-high-throughput approach based on ultra-high-performance liquid chromatography with diode array detection (UHPLC-DAD) in combination with simple syringe filtration was successfully developed and validated for the quantitation of nine synthetic colorants in beverages. The recoveries of the colorants from the beverages were found to be dramatically affected by the syringe filter membrane types and pH of the sample solution. The high recoveries of the nine colorants (92.7-105.9%) were achieved by syringe filtration with poly(vinylidene difluoride) membrane following the pH adjustment of sample solution at pH 7.0. The sample treatment procedure was very simple and took only 1 min. The fast chromatographic separation (1 min) of the nine synthetic colorants was achieved by UHPLC-DAD using a C18-core-shell column. This analytical approach (UHPLC-DAD combined with syringe filtration) took only approximately 3 min. The established method was ultrafast, sensitive, precise, accurate, and reliable. The method was successfully applied to rapidly determine the 9 colorants in 17 beverages.