Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, Biomedical Engineering Research Center, Asan Institute of Life Sciences, Asan Medical Center, 05505, Seoul, Republic of Korea.
Sci Rep. 2020 Sep 24;10(1):15592. doi: 10.1038/s41598-020-72913-8.
The early diagnosis and monitoring of cancers are key factors in effective cancer treatment. Particularly, the separation of biomolecules is an essential step for both diagnostic and analytical purposes. However, the current techniques used to isolate biomolecules are intensive, laborious, and require multiple instruments as well as repeated sample preparations to separate each biomolecule. Thus, an efficient separation system that can simultaneously separate biomolecules from scarce samples is highly desirable. Hence, in this study, we developed a biosilica-based syringe filtration system for the efficient separation of biomolecules from cancer samples using amine-modified diatomaceous earth (AD) with dimethyl 3,3'-dithiobispropionimidate (DTBP). The syringe filter can be an efficient and rapid tool for use in various procedures without complex instruments. The DTBP-based AD system was combined with the syringe filter system for nucleic acid and protein separation from various cancer cells. We demonstrated the efficacy of the DTBP-based AD in a single-filter system for the efficient separation of DNA and proteins within 40 min. This DTBP-based AD syringe filter system showed good rapidity, efficiency, and affordability in the separation of biomolecules from single samples for the early diagnosis and clinical analysis of cancers.
癌症的早期诊断和监测是癌症治疗的关键因素。特别是，生物分子的分离是诊断和分析目的的关键步骤。然而，目前用于分离生物分子的技术既密集又费力，需要多种仪器和多次样品制备来分离每种生物分子。因此，非常需要一种能够从稀有样本中同时分离生物分子的高效分离系统。因此，在这项研究中，我们使用胺修饰的硅藻土（AD）和二甲基 3,3'-二硫代双丙酰胺（DTBP）开发了一种基于生物硅的注射器过滤系统，用于从癌症样本中高效分离生物分子。注射器过滤器可以作为一种高效、快速的工具，无需复杂的仪器，即可用于各种程序。基于 DTBP 的 AD 系统与注射器过滤系统相结合，用于从各种癌细胞中分离核酸和蛋白质。我们证明了基于 DTBP 的 AD 在单个过滤器系统中从单个样品中分离 DNA 和蛋白质的有效性，时间为 40 分钟。这种基于 DTBP 的 AD 注射器过滤系统在从单个样本中分离生物分子方面具有良好的快速性、效率和经济性，可用于癌症的早期诊断和临床分析。
