Al-Hindi Bassel, Yusoff Nor A, Atangwho Item J, Ahmad Mariam, Asmawi Mohd Z, Yam Mun F
School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11700 Minden, Penang, Malaysia.
Cluster of Integrative Medicine, Advanced Medical and Dental Institute, 13200 Penang, Malaysia.
Med Sci (Basel). 2016 Apr 25;4(2):9. doi: 10.3390/medsci4020009.
Benth. (GL) possesses considerable glucose lowering effects able to be utilized on a large-scale. This paper investigates the effects of a Soxhlet extract on hyperglycemia, Langerhans islets and glucose uptake by abdominal muscles.
Ethanol and a Soxhlet apparatus were used to obtain GL ethanolic Soxhlet extract (GLES). It was then administered to randomly-segregated male , normal and STZ-induced diabetic rats, using oral gavage to evaluate blood glucose levels (BGLs), serum lipid profile, insulin levels and the pancreas post-treatment.
GLES significantly ( < 0.05) decreased BGLs of normal rats in glucose tolerance testing at a dose of 2 g/kg b.w. but failed to do so in diabetic rats undergoing acute 7-h treatment. Given twice-daily, 1 g/kg b.w. of GLES moderately controlled diabetic BGLs starting from day 10. After 14 days of treatment, 1 g/kg and 0.5 g/kg b.w. of GLES caused 44% and 50% respective increases in the average area of Langerhans islets compared to DC. Using isolated rat abdominal muscle, GLES was found to be a mild insulin-sensitizer. GC-MS analysis revealed the presence of the known glucose-lowering phytosterol, Sitostenone.
Despite retaining moderate antidiabetic activity, Soxhlet extraction of probably leads to the destruction of active heat-liable compounds.
Benth.（GL）具有显著的降血糖作用，可大规模应用。本文研究了索氏提取物对高血糖、胰岛以及腹肌葡萄糖摄取的影响。
使用乙醇和索氏提取器获得GL乙醇索氏提取物（GLES）。然后将其给予随机分组的雄性正常大鼠和链脲佐菌素诱导的糖尿病大鼠，通过灌胃给药来评估血糖水平（BGLs）、血脂谱、胰岛素水平以及治疗后的胰腺情况。
在葡萄糖耐量试验中，2 g/kg体重剂量的GLES显著（<0.05）降低了正常大鼠的血糖水平，但在急性7小时治疗的糖尿病大鼠中未能降低血糖。从第10天开始，每天两次给予1 g/kg体重的GLES可适度控制糖尿病大鼠的血糖水平。治疗14天后，与糖尿病对照组相比，1 g/kg和0.5 g/kg体重的GLES分别使胰岛平均面积增加了44%和50%。使用分离的大鼠腹肌，发现GLES是一种轻度的胰岛素增敏剂。气相色谱-质谱分析显示存在已知的降血糖植物甾醇，谷甾烷酮。
尽管保留了适度的抗糖尿病活性，但对GL进行索氏提取可能导致活性热不稳定化合物的破坏。
