Ojo Oluwafemi Adeleke, Osukoya Olukemi Adetutu, Ekakitie Lisa Ilobekemen, Ajiboye Basiru Olaitan, Oyinloye Babatunji Emmanuel, Agboinghale Precious Eseose, Kappo Abidemi Paul
Present Address: Department of Biochemistry, Phytomedicine and Biochemical Toxicology Research Laboratory, Landmark University, Omu-Aran, Kwara State Nigeria.
Department of Biochemistry, Phytomedicine, Biochemical Toxicology and Biotechnology Research Laboratory, College of Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State Nigeria.
J Diabetes Metab Disord. 2020 May 12;19(1):469-481. doi: 10.1007/s40200-020-00533-0. eCollection 2020 Jun.
leaf is used traditionally to treat diabetes and other diseases. The present study aimed to provide the modulatory effect of on hyperglycemia, inhibitory effect of redox imbalance and inflammation in alloxan-induced nephropathy in Wistar rats.
Alloxan monohydrate was used to induce diabetes by an intraperitoneal injection of (150 mg/kg). Three diabetic groups were administered aqueous leaf extract of at 6.36, 12.72 and 25.44 mg/kg bodyweight (BW) respectively; a group was administered with metformin (5 mg/kg BW), while the other two were served as positive and negative control. Thereafter, fasting blood glucose, antioxidant enzymes, malondialdehyde (MDA) level, interleukin 2 and 6 were determined.
leaf significantly ( < 0.05) reduced the alloxan-induced increases in blood glucose, MDA, interleukin 2 and interleukin 6 level and increased the alloxan-induced decreases in superoxide dismutase, catalase, glutathione peroxidase, glutathione reduced and glutathione transferase activity. All these changes compared with those of metformin-treated diabetic rats.
The data from this study suggest that modulates glucose homeostasis as well as inhibiting redox imbalance and inflammation in diabetic rats, which may be attributed to the effects of its phytochemical constituents such as saponins, flavonoids and alkaloids. It also indicated that inhibition of inflammatory cytokines and redox imbalance are likely mechanisms by which leaf exert its antidiabetic action.
传统上该植物叶用于治疗糖尿病和其他疾病。本研究旨在探讨该植物叶对链脲佐菌素诱导的Wistar大鼠高血糖的调节作用、氧化还原失衡的抑制作用以及对炎症的影响。
通过腹腔注射一水合链脲佐菌素（150mg/kg）诱导糖尿病。三个糖尿病组分别给予该植物叶水提取物，剂量为6.36、12.72和25.44mg/kg体重（BW）；一组给予二甲双胍（5mg/kg BW），另外两组分别作为阳性和阴性对照。此后，测定空腹血糖、抗氧化酶、丙二醛（MDA）水平、白细胞介素2和6。
该植物叶显著（P<0.05）降低了链脲佐菌素诱导的血糖、MDA、白细胞介素2和白细胞介素6水平的升高，并增加了链脲佐菌素诱导的超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、还原型谷胱甘肽和谷胱甘肽转移酶活性的降低。所有这些变化均与二甲双胍治疗的糖尿病大鼠相比。
本研究数据表明，该植物叶可调节糖尿病大鼠的葡萄糖稳态，抑制氧化还原失衡和炎症，这可能归因于其植物化学成分如皂苷、黄酮类化合物和生物碱的作用。还表明抑制炎性细胞因子和氧化还原失衡可能是该植物叶发挥抗糖尿病作用的机制。
