Gorda Barbara, Toelzer Christine, Aulicino Francesco, Berger Imre
The School of Biochemistry and Bristol Synthetic Biology Centre BrisSynBio, University of Bristol, Tankard's Close, Bristol, United Kingdom.
The School of Biochemistry and Bristol Synthetic Biology Centre BrisSynBio, University of Bristol, Tankard's Close, Bristol, United Kingdom; Max Planck Bristol Centre for Minimal Biology, School of Chemistry, University of Bristol, Cantock's Close, Bristol, United Kingdom.
Methods Enzymol. 2021;660:129-154. doi: 10.1016/bs.mie.2021.06.018. Epub 2021 Jul 13.
The baculovirus expression vector system (BEVS) delivers high yield heterologous protein expression and is widely used in academic and industrial R&D. The proteins produced enable many applications including structure/function analysis, drug screening and manufacture of protein therapeutics. Vital cellular functions are controlled by multi-protein complexes, MultiBac, a BEVS specifically designed for heterologous multigene delivery and expression, has unlocked many of these machines to atomic resolution studies. Baculovirus can accommodate very large foreign DNA cargo for faithful delivery into a target host cell, tissue or organism. Engineered MultiBac variants exploit this valuable feature for delivery of customized multifunctional DNA circuitry in mammalian cells and for production of virus-like particles for vaccines manufacture. Here, latest developments and applications of the MultiBac system are reviewed.
杆状病毒表达载体系统（BEVS）可实现高产异源蛋白表达，广泛应用于学术和工业研发。所产生的蛋白质有多种用途，包括结构/功能分析、药物筛选以及蛋白质治疗药物的制造。重要的细胞功能由多蛋白复合物控制，MultiBac是一种专门设计用于异源多基因递送和表达的BEVS，它使许多此类复合物得以进行原子分辨率研究。杆状病毒能够容纳非常大的外源DNA载荷，以便将其准确递送至靶宿主细胞、组织或生物体。经过改造的MultiBac变体利用这一宝贵特性，在哺乳动物细胞中递送定制的多功能DNA电路，并用于生产用于疫苗制造的病毒样颗粒。本文综述了MultiBac系统的最新进展和应用。
