Rahman Shafkat Shamim, Siddique Romana, Tabassum Nafisa
Biotechnology Program, Department of Mathematics and Natural Sciences, BRAC University, 66, Mohakhali, Dhaka, 1212, Bangladesh.
United Surgical (BD) Ltd, Plot# 659-661, Islampur, Kadda, Gazipur, 1702, Bangladesh.
BMC Res Notes. 2017 Oct 30;10(1):531. doi: 10.1186/s13104-017-2855-7.
Halotolerant bacteria have multiple uses viz. fermentation with lesser sterility control and industrial production of bioplastics. Moreover, it may increase the crop productivity of coastal saline lands in Bangladesh by transferring the salt tolerant genes into the plants. The study focused on the isolation and identification of the halotolerant bacteria from three soil samples, collected from coastal Patenga area. The samples were inoculated in nutrient media containing a wide range of salt concentrations.
All the samples showed 2, 4 and 6% (w/v) salt tolerance. The isolates from Patenga soil (4, 6%) and beach soil (2%) showed catalase activity and all the isolates showed negative results for oxidase activity, indole production, lactose and motility. All the samples provided positive results for dextrose fermentation. Other tests provided mixed results. Based on the morphological characteristics, biochemical tests and ABIS software analysis the isolates fall within the Enterobacteriaceae, Clostridium and Corynebacterium, with a predominance of Vibrios. Overall the isolates can be considered as mild halotolerant, with the best growth observed at lower salinities and no halophilism detected. Among many possibilities, the genes responsible for the salt tolerant trait in these species can be identified, extracted and inserted into the crop plants to form a transgenic plant to result in higher yield for the rest of the year.
嗜盐细菌有多种用途，即发酵时所需的无菌控制较少以及用于生物塑料的工业化生产。此外，通过将耐盐基因转移到植物中，它可能会提高孟加拉国沿海盐碱地的作物产量。该研究聚焦于从沿海帕滕加地区采集的三个土壤样本中分离和鉴定嗜盐细菌。这些样本被接种到含有多种盐浓度的营养培养基中。
所有样本均表现出对2%、4%和6%（w/v）盐浓度的耐受性。来自帕滕加土壤（4%、6%）和海滩土壤（2%）的分离菌株显示出过氧化氢酶活性，所有分离菌株的氧化酶活性、吲哚产生、乳糖利用和运动性检测结果均为阴性。所有样本的葡萄糖发酵检测结果均为阳性。其他检测结果不一。基于形态特征、生化检测和ABIS软件分析，这些分离菌株分属于肠杆菌科、梭菌属和棒状杆菌属，其中弧菌属占主导。总体而言，这些分离菌株可被视为轻度嗜盐菌，在低盐度条件下生长最佳，未检测到嗜盐性。在众多可能性中，可以鉴定、提取这些物种中负责耐盐性状的基因，并将其插入农作物中，形成转基因植物，从而在剩余年份实现更高产量。
