Bakht Jehan, Qureshi Maryam, Iqbal Arshad, Shafi Mohammad
Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, KPK, Pakistan.
Department of Botany, Islamia College, Peshawar, Pakistan.
Pak J Pharm Sci. 2019 Jan;32(1):177-184.
Different solvent extracted samples from the leaves and fruits tissues of D. stramonium were tested against five pathogenic microorganisms by disc diffusion susceptibility method using 1, 2 and 3mg disc concentrations. Methanol and chloroform extracted fractions from both leaves and fruits measured good growth inhibition of all the tested microorganisms at all concentrations. Bacillus subtilis was very resistant to n-butanol and aqueous extracted fractions of fruits tissues at all the tested three concentrations. The growths of Pseudomonas aeruginosa and Klebsiella pnuemonia were efficiently reduced by all the solvent extracted fractions from the fruits while aqueous fraction was unable to restrain the growth of Bacillus subtilis. The growth of Candida albicans was effectively reduced by aqueous extracted fraction from the leaves tissue at the highest concentration. Maximum growth reduction of (77%) was shown by chloroform extracted fractions from the leaves against Klebsiella pneumonia at 3mg disc-1 concentration. Minimum zone of inhibition (35.4%) was measured by n-butanol extracted fractions from the leaves against Pseudomonas aeruginosa at the lowest concentrations of 1mg disc. In case of leaves the most vulnerable bacteria was Bacillus subtilis while in case of fruits Klebsiella pneumonia was the most susceptible while Bacillus subtilis was the most resistant one.
采用纸片扩散法，使用1mg、2mg和3mg的纸片浓度，对曼陀罗叶和果实组织的不同溶剂提取物样本进行了针对五种致病微生物的测试。叶片和果实的甲醇提取物和氯仿提取物在所有浓度下对所有测试微生物均表现出良好的生长抑制作用。在所有测试的三种浓度下，枯草芽孢杆菌对果实组织的正丁醇提取物和水提取物具有很强的抗性。果实的所有溶剂提取物均能有效抑制铜绿假单胞菌和肺炎克雷伯菌的生长，而水提取物无法抑制枯草芽孢杆菌的生长。叶片组织的水提取物在最高浓度下能有效抑制白色念珠菌的生长。叶片的氯仿提取物在3mg纸片浓度下对肺炎克雷伯菌的生长抑制率最高，达77%。叶片的正丁醇提取物在1mg纸片最低浓度下对铜绿假单胞菌的抑菌圈最小，为35.4%。就叶片而言，最易受影响的细菌是枯草芽孢杆菌；就果实而言，肺炎克雷伯菌最敏感，而枯草芽孢杆菌最具抗性。
